Time-lapse movies acquired using Incucyte® Live-Cell Analysis System showing a T cell sub population of PBMCs, activated with anti-CD3 antibody and IL-2, killing target SK-OV-3 tumor cells. Labeling of the SK-OV-3 cancer cells with the Incucyte®️ Nuclight Red Lentivirus. Incucyte®️ Cell-by-Cell Analysis Software was then used to quantify and graph total target and effector cell proliferation and apoptotic index when Nuclight Red Ramos cells were cultured with activated human PBMCs in the presence of Incucyte®️ Annexin V Green Reagent. Finally, Nuclight Red A549 spheroids were cultured with non-activated and activated PBMCs to study immune cell killing in a 3D model.