Successful optimization strategies utilizing Design of Experiments (DoE) have increased the yield of AAV2 to over 95%. The use of Sartoclear Dynamics®️ Lab Filter Aid reduces manual handling time 3-fold and increases the filter capacity 3.5-fold compared to the standard laboratory method of filtration combined with a prior centrifugation step. Learn how to reduce turbidity, increase filter capacity, and decrease manual handling in this application note. What You Will Learn ■ Why Sartoclear Dynamics®️ Lab filtration is a viable approach to clarifying AAV crude cell lysates ■ The impact of Sartoclear Dynamics®️ Lab on turbidity removal, AAV) yield, filter capacity and manual handling times. ■ The effect of diatomaceous earth (DE) concentration and DE incubation time on AAV2 retention and turbidity removal in a DoE study