Ambr® 15 Cell Culture
Ambr® 15 Cell Culture is a high throughput, automated bioreactor system for 24 or 48 parallel cultivations at the 10 –15 mL microbioreactor scale. This platform enables selection of the best clones, media and feeds under small-scale bioreactor conditions, ensuring a seamless scale-up. Implemented in Cell Line Development (CLD) laboratories worldwide, Ambr® 15 Cell Culture makes experimentation more cost effective by saving on labor, facility space, capital, media and consumables.
Leave shake flasks and shaking plates behind to increase cell productivity and ensure robust performance.
With up to 48 bioreactors running in parallel one operator can screen more clones and conduct multi-factor DOE studies.
Single-use bioreactors save setup time, reducing staff costs and turnaround time.
Liquid handling automation reduces variation and risk of human error.
A one-year license for Ambr® Clone Selection software is included, simplifying ranking analysis.
The Ambr® 15 Cell Culture system includes single-use vessels, an automated workstation and powerful software. Installed in a biosafety cabinet for aseptic operation, Ambr® 15 monitors and controls 24 or 48 microbioreactor cultures in parallel to provide a reliable microscale model for upstream processes. Implemented in CLD workflows of major pharma and biologics companies as well as research and academic institutes.
With an improved design, this next generation system offers better performance, increased process flexibility and expanded capabilities to support a wide range of upstream applications.
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Fully automated parallel processing, monitoring and control with:
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The culture stations are located on the workstation deck: two culture stations in a 24-way system, four in a 48-way system – each station holds 12 microbioreactors.
Temperature and stirring speed are consistent within a single culture station but can vary between different culture stations.
pH, DO, gassing, liquids feeds and samples are controlled independently for each individual bioreactor within a culture station.
Ambr® 15 microbioreactor vessels mimic the characteristics of lab scale bioreactors to enable optimal cell growth, productivity and product quality.
Ambr® 15 control software supports:
The Ambr® 15 software includes a license for MODDE® DOE to assist with experiment planning and execution.
Process parameters and other variables can be tagged in Ambr® 15 to facilitate DOE, enabling easy transfer and analysis of results in MODDE®.
Ambr® 15 Cell Culture is a microscale bioreactor model that reduces bottlenecks and accelerates development across a range of upstream applications. With the ability to efficiently explore different modes of operation, it is easy to investigate how cell lines and clones behave under batch, fed-batch and perfusion-mimic conditions, while the capability of media exchanges supports development of processes for cell and gene-based therapies.
Assessing clone performance in small-scale bioreactors ensures results translate at scale. Ambr® 15 Cell Culture combined with Ambr® Clone Selection for clone ranking optimizes workflows to reduce cell line development timelines and increase productivity.
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Optimization of media and feeds can improve cell growth and productivity. Ambr® 15 Cell Culture control software enables the creation of media blends in plates on the deck or directly in the microbioreactors. Automated liquid handling saves time and reduces risk of error.
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Protocols can rapidly be established for both stable and transient cell lines. Parallel operation allows testing of multiple factors to define process parameters early in development, while Design of Experiments (DOE) embedded in the Ambr® 15 software facilitates the multifactorial experimental design and analysis.
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Ambr® 15 Cell Culture’s Rapid Vessel Drain option removes supernatant from settled cultures directly from the microbioreactors quickly and efficiently. The Ambr® 15 software includes novel process steps, such as cell bleeding based on cell count data, to automate perfusion mimic protocols.
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Developing novel, complex processes quickly is essential to manufacture products on time and to specifications. Ambr® 15 Cell Culture helps to establish protocols for stable and transient cell lines, select suitable clones, and optimize media and processes to facilitate scale up to larger bioreactors.
Evaluate and compare performance of CHO clones in a standard platform process or screen media and feed compositions in parallel.
Define small scale processes and optimize transfection conditions for suspension-adapted HEK293 and HEK293T cells.
Transition from 2D-to-3D culture, and utilize high throughput experiments to optimize CPPs for workflow development of iPSCs and derivatives.
Support T-cell and CAR-T cell expansion processes at the smallest bioreactor scales for efficient adaptation to a stirred environment.
Integration of the Analysis module with Ambr® 15 for at-line pH measurements automates bioreactor sensor calibration and in-process pH checks, while the Analysis module with BioPAT® Spectro allows connection to selected 3rd party Raman Spectrometers for spectroscopic analysis of bioreactor samples.
The Ambr® 15 Cell Culture system can connect directly to the Beckman Vi-CELL™ XR or Roche Cedex HiRes for cell count measurements, or to the Nova Biomedical BioProfile® FLEX2 for cell counts and metabolite analysis. Samples transfer through a sample line to the integrated analyzers, and measurement results transfer back to the Ambr® 15 software for monitoring and process control.
The Octet® Label-free Detection Systems and Waters™ BioAccord™ LC-MS can connect to Ambr® 15 to transfer bioreactor sample data to the analyzer and return results to the Ambr® 15 software. Samples are manually transported between the systems.
Click the signs to learn more about the features that make the Ambr® 15 Cell Culture the bioreactor system of choice for Cell Line Development laboratories worldwide. This technology enables the selection of the best clones, media and feeds under small-scale bioreactor conditions for a seamless scale-up, all while saving on labor, facility space, capital expenditures, media and consumables.
The optional Rapid Vessel Drain facilitates removal of large volumes of culture or spent media from a bioreactor in a single step via a dedicated pipette mandrel and vessel drain assembly. Applications include passaging of cultures, media exchanges and performing perfusion mimic processes.
pH and DO card readers under the deck monitor single-use sensor spots in the Ambr® 15 microbioreactors. Control is applied through the supply of individual gas mixtures and base additions to maintain target setpoints.
The large tip bin, interchangeable with the small tip bin, complements the increased tip capacity offered by the Flexible Deck, allowing a longer run-time without operator involvement. The tip bins easily disconnect from the system for emptying and cleaning.
Four culture stations operate up to 48 cultures in parallel, with a new design that improves temperature performance and stirrer speed control. Individual gassing lines deliver independent gas mixtures to each microbioreactor.
Flexible positions for pipette tip boxes, plates, tip box lids and plate lids allow operators to define setup and then change labware configurations during the process as needed, with six flexible labware positions on the 24-way system and nine on the 48-way system.
The new Liquid Handler design improves dispense accuracy during automated pipetting of media, feed and reagents into microbioreactor vessels or labware on the deck, sampling from microbioreactors into the sample cup for integrated at-line analysis, or sampling into plates for off-line analysis.
Ambr® 15 microbioreactors have a recommended working volume of 10 – 15 mL for optimal performance. Culture volumes typically vary during a process from feed and base additions and sample removal.
Single-use sensor spots in the microbioreactors allow online monitoring of individual pH and DO levels in cultures during the process.
Integrated pitched blade impellers , similar to larger traditionally stirred mammalian bioreactors (e.g., Ambr® 250 and Biostat STR®) provide effective mixing without damaging cells.
The liquid handler picks up and replaces caps on the sample port. Liquids are added using the 1 mL or 5 mL pipette tips while samples are removed using the 1 mL pipette tip.
The gas sparge tube provides direct gassing into the culture liquid to support the oxygen demand of insect and mammalian cell lines. For more sensitive cell lines, vessels are available instead that provide headspace gassing.
Explore brochures, posters and application notes on Ambr® 15 for a full understanding of how the lab-scale bioreactor can increase productivity while reducing timelines across a number of applications.
Find out more about processes conducted in Ambr® 15 systems. The bibliography lists publications by phase in the process stream.
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