Analyzers for Ambr® | Sartorius

Analyzer Technologies Compatible with Ambr® 15 and Ambr® 250

Integrated and Stand-Alone Analyzers for Key Cell Culture Process Parameters

Ambr® systems have been developed with compatibility to a range of bioprocess analyzer technologies. This can be via integration to the Ambr® system, or via automated transfer of bioreactor sample data and return of analytical results.

Integrated analyzers allow routine measurement of key cell culture process parameters including cell viability, viable cell density and metabolite levels. Analyzers with data-connectivity provide critical quality attribute (CQA) information including product titer, glycosylation levels and cell culture media composition.


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Integrated Analyzers

Integration of proven technologies into Ambr® systems

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Data Connected Analyzers

Stand-alone analyzers with data connection to Ambr® systems

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Ambr® Analyzer Options

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Compare Analyzers Compatible with Ambr® Systems

Ambr® 15 and Ambr® 250 High Throughput systems support a wide range of bioprocess analyzers.
 

Ambr® Analysis Module

Octet® BLI

BioAccord

BioProfile® FLEX2

Vi-CELL BLU Analyzer or Cedex HiRes

Raman Rxn2* or HyperFLUX PRO*

Primary Application

pH calibration

Titer | binding kinetics

Protein | subunit measurement

Metabolite analysis

Viable cell count

Scale down model building

Secondary Application

Spectroscopy

Glycan analysis

Media analysis

Viable cell count

 

Process transfer across scales

Connects with Ambr® 15

 

 

 

 

 

 

Ambr® 250 High Throughput

 

 

 

 

 

Direct Integration

 

 

 

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Data-Connectivity

 

 

*Analyzer connected via the Ambr® Analysis Module

Integrated Analyzers

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Ambr® Analysis Module

Ambr® Analysis Module automates pH sensor calibration and BioPAT Spectro applications.

Fully integrated into the Ambr® system, bioreactor samples are pipetted to the Analysis Module and results returned to the Ambr® software alongside bioreactor process data.

  • Initial bioreactor vessel pH sensor calibration
  • Analysis temperature matched to bioreactor temperature
  • Automated in-process, pH sensor recalibration

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Endress+Hauser Raman Rxn2

The Raman Rxn2 automates Raman spectroscopy.

Raman Rxn2 integration is achieved via the BioPAT® Spectro option available for the Ambr® Analysis Module, which transfers bioreactor samples to a flow cell and results returned to the Ambr® software alongside bioreactor process data.

  • Supports QbD high-throughput process development
  • Enables scale down model building
  • Consolidation and contextualization of spectral data with Ambr® bioprocess data

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Tornado Spectral Systems HyperFlux PRO 785

The HyperFlux PRO 785 automates Raman spectroscopy.

HyperFlux PRO 785 integration is achieved via the BioPAT® Spectro option available for the Ambr® Analysis Module, which transfers bioreactor samples to a flow cell and results returned to the Ambr® software alongside bioreactor process data.

  • Supports QbD high-throughput process development
  • Enables scale down model building
  • Consolidation and contextualization of spectral data with Ambr® bioprocess data

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Nova Biomedical BioProfile® FLEX2

The FLEX2 automates metabolite analysis.

Fully integrated into the Ambr® system, the FLEX2 manages bioreactor samples processing and returns metabolite data to the Ambr® software alongside bioreactor process data.

  • Fully automated sample processing, analysis, and reporting
  • Individual metabolite data can be used in bioreactor control processes, e.g. glucose additions triggered by low glucose readings
  • Simplified data management workflow reduces errors

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Roche Cedex HiRes

The Cedex HiRes automates viable cell counting.

Fully integrated into the Ambr® system, bioreactor samples are pipetted to the Cedex HiRes and results returned to the Ambr® software alongside bioreactor process data.

  • Provides total viable cell density and percentage viability
  • Allows cell counts to be performed overnight and at weekends
  • Results can be used for process control, e.g. samples for product analysis taken once target cell densities are reached

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Vi-CELL BLU Analyzer

The Vi-CELL BLU Analyzer automates viable cell counting.

Fully integrated into the Ambr® system, bioreactor samples are pipetted to the Vi-CELL BLU Analyzer for analysis and results returned to the Ambr® software alongside bioreactor process data.

  • Provides total viable cell density and percentage viability
  • Results can be used for process control, e.g. feed additions calculated and executed based on cell density
  • Enables cell counts to be made out of hours without operator attendance

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Data Connected Analyzers

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The Octet® BLI is an easy-to use, real-time analytical tool that rapidly screens critical quality attributes.

Octet® BLI Label-free Detection Systems

The Octet® BLI automates titre and glycan analysis.

Octet® BLI support is achieved via data interfaces facilitating transfer of bioreactor sample data to the analyzer and return of results to the Ambr®. Samples are manually transported between the systems.

  • Rapid titer measurement
  • Analytes can be measured in crude, unpurified samples  
  • Relative protein glycosylation levels

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Waters™ BioAccord LC-MS

The BioAccord™ allows multi-attribute analysis of biological molecules.

BioAccord™ support is achieved via data interfaces facilitating transfer of bioreactor sample data to the analyzer and return of results to the Ambr®. Samples are manually transported between the systems.

  • Automation of data acquisition, processing and reporting of complex analytical workflows
  • Reduced dependency on core analytical labs
  • Analysis of product attributes and cell culture media

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Data Analytics

Flexibility – Performance – Quality Assurance

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Ambr® Clone Selection powered by Umetrics®

Standalone application designed for use with data from Ambr® systems to simplify the workflow for cell line screening and ranking.

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MODDE® Design of Experiment software

The fastest and most cost-efficient way to design effective experiments, increase productivity and de-risk projects.

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Poster

Investigating the Use of Automation and Integrated Analytics to Enhance Cell Line and Process Development Capabilities

PDF | 2.6 MB
Poster

Automated Glucose Control in Ambr® Bioreactors Using Raman Spectroscopy

PDF | 1.9 MB
Poster

Facilitated Process Scalability Using a Novel Raman Spectroscopy Integration

PDF | 853.7 KB
Application Note

In-Process Media Monitoring Using the BioAccord™ LC-MS System for the Automated High Throughput Multi-Parallel Ambr® 15 Microbioreactor System

PDF | 546.1 KB

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Related Products

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Ambr® 15 Cell Culture

Automated microbioreactor system for cell line development and process optimization, with 24 or 48 single-use 10-15mL microbioreactors.

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Ambr® 250 High Throughput

High throughput, automated bioreactor system for process development with 12 or 24 single-use 100–250mL mini bioreactors.

Explore Ambr® 250 High Throughput


Biostat STR®

A range of Analytics and Sensors for Uni-/ Multi-variate Data Gathering: Real Time Monitoring & Control.

Explore Biostat STR®

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