Biostat® D-DCU - Compact Bioprocess System | Sartorius

Biostat® D-DCU

Your Fast Lane To Production

The Biostat® D-DCU is a compact bioprocess system available in microbial or cell culture versions with vessel choices from 10 to 200L working volume. The optimized and proven design of the Biostat® D-DCU is the result of thorough analysis of the most required features and functions from over thirty years of stainless steel fermenter | bioreactor design experience. This standardized solution eliminates design times, allows faster delivery, reduces cost, guarantees trouble free operation, allows for global service support as well as spare part availability.

Top Features

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  • Designed to interface single-use storage bags for media addition and harvesting, as well as the TAKEONE® single-use aseptic sampling systems
  • Enhanced functionality and ease of use are provided by a wide selection of in-line sensors and analyzers and by integrated display of process values on the DCU screen

  • Due to the modular design, various configurations are available -  from basic to fully featured
  • The system offers an excellent solution for any budget and every need
  • Automated Sterilization in Place (SIP)
  • Automated Cleaning in Place (CIP)

  • Scalable BioPAT® MFCS S88 recipes provide a significant increase in process safety and reliability and result in improved batch-to-batch consistency

Discover the Biostat® D-DCU

Innovative Technology for Your Process Needs

  • Process development and scale-up
  • Seed production
  • GMP production

Working Volumes are available in a choice of (2:1 or 3:1 diameter ratio)

  • 10 L
  • 20 L
  • 30 L
  • 50 L
  • 100 L
  • 200 L
  • BioPAT® Trace - Glucose | Lactate measurement
  • - Turbidity measurement
  • - Off-gas analysis
  • - Multi-variant bioprocess monitoring
  • Learn more about BioPAT® Sensors

Gassing Strategies

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O2 Enrichment

This is a gassing strategy uses a 3 | 2-way solenoid valve to direct either an air or O2 flow to the sparger. A manual flow meter visually indicates and controls the flow rate. O2 is pulsed via solenoid valve, when required to maintain the dissolved oxygen setpoint. Air is not provided at this time. You can optionally integrate mass flow controllers to measure and control the total gas flow rate via manual adjustment or automatically in conjunction with the controller, based on the signal of the pO2 probe and the selected setpoint.

Advanced Additive Flow

You can direct air, O2 , N2 and CO2  to the sparger and to the overlay. Flow meters visually indicate the flow rate for each gas. Add an additional gas flow path to the sparger or overlay outlet. Select optional mass flow controllers for each flow path, and switch gases between overlay and sparger. The detailed design of the Advanced Additive Flow gassing approach depends on your particular Biostat® bioreactor system and configuration.

Additive Flow

Controls the flow rate of air and O2 individually, (N2 and CO2 also possible but not shown) to a single output, either a sparger or overlay.

  • Microbial and cell culture
  • Suspension and microcarrier cultures

Performance

High-cell density

Employing the Biostat® D-DCU 10-3 it is demonstrated in this study, that this fermenter meets the requirements for an E. coli high-cell density fermentation.

Standardized Qualification of Biostat® D-DCU

The performance capabilities of a bioreactor, which are substantially influenced by its design and thus, its bioengineering characteristics, are crucial in order to choose a system matching the specific process requirements.

In order to guarantee a reliable comparison between existing bioreactor systems the DECHEMA guideline was published by an expert group as a standardized method required for the determination of the key bioengineering data.

Here the Biostat® D-DCU 30-3 and 100-3 are characterized according to this recommendation.

Standardized Qualification of Stirred Bioreactors for Microbial Biopharmaceutical Production Processes

Brochure

Biostat® D-DCU - Your “Fast Lane” To Production

PDF | 2.4 MB
Presentation

Biostat ® D DCU - Your “Fast Lane” to Production

PDF | 2.7 MB
Application Note

High-cell-density Cultivation of Escherichia coli in a Biostat® D-DCU 10-3 Stainless Steel Bioreactor

PDF | 572.6 KB

Consult Our Experts

Consult Our Experts

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