A Robust Portfolio of Resins
A Robust Portfolio of Resins Offering High Selectivity, Flexibility, and Productivity
Sartorius chromatography resins encompass ion exchange, affinity and mixed mode chemistries coupled with resin beads that offer high flow and superior purification capabilities. Use of our salt-tolerant or mixed-mode resins can lead to significant time and cost savings by eliminating dilution and adjustment steps. Sartorius resins are industry proven to perform packing and purification from benchtop to commercial scale, with several formats to accommodate screening through commercial production needs.
Use of salt-tolerant resins reduce or eliminate the need for dilution or adjustment steps
Unique mixed-mode ligands offer powerful separation capability for the most complex purification challenges
Industry-proven packing and purification performance from process development to commercial scale production
Sartorius chromatography resins deliver high purity within a simplified process design for small and medium-sized biomolecules. Our resins offer flexible process integration without the need for feedstream manipulation. Our broad portfolio covers our customers’ process requirements and addresses their purification challenges. Our unique selectivity with mixed-mode ligands for both capture and purification will increase the productivity of the existing standard purification platforms.
IEX
Affinity
Mixed-Mode
Ligands
Q, DEAE, CM, STAR AX
Heparin, Lysine, Blue Dye
CMM, HEA, PPA, MEP, HA
Common applications
mAbs, recombinant proteins, viruses/VLPs, blood fraction, nucleic acids
Recombinant proteins, viruses/VLPs, blood fraction
mAbs, recombinant proteins, fabs, bispecific Abs
Resin beads
HyperCel, HyperD®
HyperD®, Trisacryl®
HyperCel, Ultrogel®
Formats
Bulk resin, PRC pre-packed columns
Bulk resin
Bulk resin, pre-packed columns
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Resin
Base Matrix
Ligand
Bead Size
Binding Capacity
Q, DEAE, CM HyperD® F
Ceramic polymer composite
Ion exchange
50 µm
85 mg/mL* (AEX)60 mg/mL** (CEX)
HyperCel™ STAR AX
Rigid cellulose
Salt-tolerant anion exchange
80 µm
100 mg/mL*
HEA, PPA HyperCel™
Mixed-mode
80 - 100 µm
40 – 60* mg/mL
MEP HyperCel™
80 – 100 µm
20 mg/mL**
CMM HyperCel™
50 – 80 µm
50 – 100** mg/mL
HA Ultrogel®
HA agarose composite
Mixed-mode (hydroxy-apatite)
60 – 180 µm
7 mg cytochrome C /ml
Blue Trisacryl® M
Trisacryl
Affinity blue dye
40 – 80 µm
10 mg/mL*
Heparin HyperD® M
Polymer composite
Affinity heparin
25 mg ATII/mL
Lysine HyperD®
Affinity lysine
70 µm
-
* Binding capacity is related to BSA** Binding capacity is related to IgG
Ion exchange resins are used in the capture and purification chromatography steps of various biomolecules. Sartorius offers a comprehensive range of chemistries and bead matrices. The resins provide high capacity at short residence times in a flexible range of conductivities up to 12 – 15 mS/cm. Unique salt-tolerant matrices, like HyperCel Star AX anion exchange resin, allow simple process integration without the need for additional feedstream manipulation.
Q Ceramic HyperD®
HyperCel STAR AX
DEAE Ceramic HyperD®
CM Ceramic HyperD®
Quaternary amine
Primary amine
Diethylaminoethyl
Carboxymethyl
IEX type
Strong anion exchange
Weak salt-tolerant anion exchange
Weak anion exchange
Weak cation exchange
Working conductivity
4-8 mS/cm
Salt tolerant: 2-15 mS/cm
Salt tolerant: up to 10-19 mS/cm
Bead material
Ceramic polymer composite (50 µm)
Rigid cellulose(80 µm)
DBC at 10% breakthrough
BSA ≥85 mg/mL
BSA ≥100 mg/mL
IgG ≥60 mg/mL
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Q Ceramic HyperD® is a strong anion exchanger that is suited for the removal of anionic impurities (i.e., host cell protein and DNA). Its 50 µm base bead offers a high resolution for purification applications when required.
HyperCel Star AX is a salt-tolerant anion exchanger with a primary amine as functional group. It can easily be integrated into a purification workflow without feedstream manipulation.
DEAE Ceramic HyperD® is a weak anion exchanger for purification. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.
CM Ceramic HyperD® is a weak cation exchanger for polishing applications. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.
Sartorius offers multiple affinity chromatography resins with high binding capacities in a variety of selectivities to purify recombinant proteins, albumin, enzymes coagulation factors (ATIII), plasminogen and growth hormones.
Heparin HyperD®
Applications
Coagulation factors (ATIII), growth hormones and enzyme purification
Plasminogen, recombinant protein and glycoprotein purification
Albumin, enzymes, recombinant proteins and purification
Dynamic binding capacity (10 % BT)
> 25 mg/mL
> 10 mg/ml
Average particle size
60 µm
Working pH range
3 –13
1 – 10
Operating pressure
<3 barg (44 psig)
Heparin HyperD® M resin is suited for the purification of heparin-binding proteins like coagulation factors, growth hormones, lipoproteins, and DNA/RNA processing enzymes.
Lysine HyperD® affinity resin is used for the purification of lysine-binding proteins such as plasminogen. It provides high protein-binding capacities at high flow rates.
Blue Trisacryl® M is an affinity resin used for the purification of enzymes, interferons, and some coagulation factors as well as for the removal of albumin. The resin uses Trisacryl® based beads which carry the blue dye ligand.
Novel biopharmaceutical targets such as nanobodies, plural-specific mAbs, and fusion proteins require unique selectivities to achieve high product purity. Mixed-mode resins leverage multi-modal separation mechanisms such as IEX and HIC to achieve higher selectivity and purity in a single step than in traditional single-mode chromatography.
Sartorius offers a broad range of selectivities to address challenges with a high concentration of contaminants with the intensified feedstreams, aggregates issues with newer modalities, and to remove challenging process or product-related impurities. Achieve reduced process costs by streamlining pre- or post-diafiltration steps.
CMM HyperCel
MEP HyperCel
HEA HyperCel
PPA HyperCel
Modes
CEX
HIC
Hydrophobic chargeinduction (HCIC)pseudo-affinityto IgG (thiophilic)
AEX
Pseudo-affinity
Application
B/E antibodyHCP, aggregate removalrecombinant molecule purification
Capture of antibodies in physiological conditions (pseudo affinity) only for MEP HyperCel; HCP, aggregate, variants, removalantibody, fab, enzymes, recombinant proteins
Dynamic binding capacity
IgG>60-100 mg/mL*
Human IgG>20 mg/mL*
BSA40-60 mg/mL*
Cytochrome C>7 mg/mL**
Average particle size (µm)
50-80
80-100
60-180
* 10% BT** 50% BT
CMM HyperCel is a hydrophobic cation exchanger recommended for the capture of antibodies, antibody fragments, and recombinant proteins. It allows the separation of proteins with similar pI by modulation of pH and conductivity.
MEP HyperCel provides unique selectivity for monoclonal antibody capture or purification to remove aggregates, HCP, and DNA. The ligand facilitates the binding of target proteins at neutral pH under moderate salt concentrations.
HEA HyperCel is a mixed-mode ligand with a less hydrophobic hexylamine chain allowing protein binding at a moderate salt concentration under neutral pH.
PPA HyperCel exhibits a stronger hydrophobicity with an aromatic ligand, allowing protein binding at a moderate salt concentration under neutral pH.
HA Ultrogel® hydroxyapatite is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed-mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. It is ideally suitable for general impurity removal.
Process intensification is an ideal solution to solve downstream chromatography bottlenecks. Learn how small and easy-to-implement changes can significantly improve productivity.
Validate the viral reduction performance of Virosart® HF with our Confidence® Services: Highly purified viruses and expert knowledge.
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