Functional Profiling | Sartorius

Functional Profiling

Phenotype and function assays are critical for selecting the top clinical antibody candidates with the desired characteristics.

The iQue® High-Throughput Screening (HTS) by Cytometry Platform supports cell-based assays, by detecting target proteins in their native conformation on the cell surface, which is a better indicator of in vivo behavior.

The iQue® HTS Platform lets you perform rapid multiplexed analysis of immune cell function through simultaneous analysis of cell-mediated cytotoxicity, cell health, function, activation, phenotyping and cytokine profiling.

Epitope Mapping: Rapidly detect and visualize epitope regions for hundreds of clones with high sensitivity, and validate your data with additional information on cell health and viability— all in one assay.

ADCC Assays: Discriminate between effector and different target cell populations in the same sample using multiplexed screening assays.


Functional Profiling Research

Featured Resources

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Poster

ADCC Poster

Rapid Multiplexed Profiling of Antibody Dependent Cell Cytotoxicity

Application Note

A High Throughput Multiplexed Antibody Internalization Assay

A high throughput, multiplexed, no wash assay was used to simultaneously measure antibody internalization, antibody specificity, and cell health.

Related Research

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Octet mAB

Maximize Efficiency in Lead Molecule Selection and Optimization

Lead selection and optimization is a crucial step in identifying molecules that meet predefined requirements and can be advanced to the next stage of development.

Octet® BLI systems are revolutionizing the speed and efficiency of selection and optimization workflows with their high-throughput and easily developed assays. These systems are ideal for applications such as affinity ranking, epitope binning, Fc-receptor binding, glycosylation screening, antibody-antigen specificity, binding characterization, and titer analysis.

With Octet® BLI systems, lead selection and optimization can be performed with greater accuracy and speed, leading to faster drug development timelines and improved outcomes.

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Resources

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Application Note

Pharmacological Characterization of the Antibody Drug Conjugate, Kadcyla®, Using Live-Cell Analysis

Application Note

iQue® Mouse IgG Type and Titer Assay Kit

 A Novel Solution to Expedite Antibody Discovery

iQue®️ High-Throughput Cytometry Platform Brochure

The iQue® 3 High-Throughput Screening Cytometry Platform allows researchers to acquire and analyze high content, multiplexed assays.

Mouse IgG TNT Fred Hutch Application Note

Researchers at Fred Hutch Cancer Center streamlined their antibody discovery workflow by utilizing the iQue® Mouse IgG Type and Titer Assay Kit t...

Featured Products

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iQue 3 and computer monitor

iQue®️ HTS by Cytometry Platform

Accelerate your research by resolving complex cellular research questions via multiplexed analysis in a single well.

Octet® BLI Label-Free Detection System instruments

Octet® BLI Label-Free Detection Systems

Enable real-time, label-free analysis for the determination of kinetics, affinity and antibody/protein quantitation.

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