Ksep® Scalable Single-Use Centrifugation System
Ksep® systems are automated, single-use solutions designed to minimize contamination risks by reducing the need for manual handling. Ksep offers scalable counterflow continuous processing for aseptic cell concentration, wash, and harvest in cell therapy manufacturing, supporting high recoveries. By harmonizing a balance between centrifugal and fluid flow forces, it imparts very low shear on cells, ensuring high viability and yield. The automated cell wash feature enhances harvest quality in cell processing applications. The Ksep® system is adaptable from process development (0.1 L) to commercial manufacturing (2,000 L), providing seamless scalability.
Ksep® is a single-use platform designed for closed processing. Ksep® systems offer dual functionality, catering to both cell harvesting (for advanced therapies) and cell removal (clarification).
Our Ksep® portfolio seamlessly scales from early development and research to cGMP production, accommodating bioreactors of up to 2,000 L. Its gentle processing capabilities enable cell recoveries of > 90% without compromising viability across the broad range of cell densities.
Ksep® 50
0.1 L – 20 L
10 L - 200 L
Up to 10 Billion Cells
Up to 80 Billion Cells
Up to 1,200 Billion Cells
50mL (2x25mL)
400 mL (4x100mL)
6000 mL (6x1000mL)
48 L/hr
114 L/hr
720 L/hr
2000 g
1000 g
The Ksep® 50 is a low-shear counterflow single-use centrifuge that delivers exceptional viability and recovery within an automated closed system.
Explore Ksep® 50
The Ksep® 400 is a closed, scalable counterflow centrifugation system that provides a compact single-use bioprocessing solution.
Explore Ksep® 400
The Ksep® 6000S is designed to continuously recover supernatant or solids while applying minimal shear on cells, resulting in unparalleled cell recovery and high throughput.
Explore Ksep® 6000S
Patented Ksep® Systems technology is the only scalable technology that provides significant advantages for users who want to either harvest cells as a product (advanced therapy manufacturing) or discard cells as a byproduct (mAb manufacturing).
Ksep® Systems are fully automated and designed to recover > 90% of the product during cell harvest and > 95% of the product during clarification applications by efficient product displacement. Low-shear processes ensure reduced downstream contamination due to cellular debris or proteolytic enzymes and high product quality. Ksep® Systems have successfully been implemented for recombinant protein (e.g., mAb) manufacturing using CHO cell culture-based processes.
Ksep® centrifuges offer the benefit of concentrating cells while maintaining high recovery and viability. These systems have a built-in wash function that can remove cell debris, dead cells, and light particulate impurities in less than five minutes. Sartorius systems can process various cell types (e.g., MSC, iPSC, NK cells, T-cells, CAR-T cells) for advanced therapy manufacturing.
Ksep® centrifuges retain particles, such as cells or microcarriers, in a concentrated fluidized bed under a continuous flow of media or buffer. Through a harmonious combination of centrifugal force and fluid flow, the system minimizes shear forces on cells, addressing prevalent concerns associated with traditional centrifugation and filtration methods. This innovative approach accommodates a broad range of cell densities, from low to very high, while ensuring high recovery and product quality.
Traditional centrifugation methods produce high shear forces that often damage cells during cell processing in advanced therapies. The Ksep® systems use advanced counterflow centrifugation, which reduces shear forces and the potential for cell lysis, resulting in superior viability and greater recovery. It reduces the need for manual intervention and offers seamless scale-up from PD to commercial manufacturing, through an automated, single-use, closed, system, that protects cells and cell products from the risk of contamination, while maintaining high cell viability and quality.
Trust our comprehensive range of services for your Bioprocess: We gladly assist you with tasks like system installation, process optimization and many...
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