Incucyte^® Service & Support

The Incucyte^® is two major components, one is the controller which is a Windows 10 computer with specialized hardware and software that controls the second component which is the gantry, the gantry moves around in x/y/z and images cells over time.

You will need the appropriate Incucyte^® GUI software version. Install the latest, and the software will let you know if you need a different version and include an appropriate link for download.

You will need to enter the IP address or hostname of the Incucyte^® to connect. The IP address is found on the Incucyte^® Controller touchscreen under “information”, make sure to click the refresh button to see the latest. The hostname can also be used. The instrument host name is by default the instrument serial number#, so this could be “Zoom4xxxx” or “ICxxxxx”, you will find your instrument serial number# on the right side of the controller.

Yes, see the standalone guide. 

No, the software only works with Windows 10 64-bit.

Yes, so long as your VPN connects you to the network where the Incucyte^® resides, you can also connect to the Incucyte^®. Note that there are usually performance issues with using a VPN due to speeds/location and this can cause errors in the software if bandwidth/speed is reduced while running the VPN.

Yes, however you need to archive the data first. Archiving is done manually through the GUI software, recommend every month, no more than 100GB at a time. You can read more about archiving in the user manual: While connected to the GUI software, go to the top of the interface and click Help->User Manual. After you have archived a scan you can safely delete it by visiting Administer->Delete.

Collect the instrument the logs and send them to our Support Team at: Incucyte.Support@Sartorius.com

For Zooms, visit Administer->Logs->Export in the GUI.

For SX5/S3/SX1, visit Device->Support->Export All.

Collect the instrument logs and send them to our Support Team at Incucyte.Support@Sartorius.com

To silence the alarm, on the Incucyte^® Controller, visit Utilities>Down Arrow>RAID>Silence Alarm>Go> Yes.

Zoom:

Try a motion calibration in that position

Follow the instructions in the User Manual.  Simply click [Run] for whichever calibration you wish to perform, and it runs based upon the objective listed under “Device status” at the bottom left of the interface.

SX5/S3/SX1 Motion Calibration: S3 Motion Calibration:

Connect to the device from the GUI and visit Device>Calibration Tests>Spatial->Check>Confirm Test and select the position you wish to calibrate. Click Yes once the Calibration Tray and Calibration Slide are loaded into the appropriate position.

The Incucyte^® Software provides four levels of user access: Admin, Standard, Limited, and Guest. The Admin account has full control of the system, whereas the Guest account (ver. 2020C and later) is a strictly Read-Only account. We recommend at least one Admin account per Incucyte^® and that regular users are given Standard access.

A comprehensive list of user permissions within the Incucyte^® Software is available in the Incucyte^® Live-Cell Analysis Software User Manual.

4x | NA 0.2, 10x | NA 0.3, 20x | NA 0.45

No, focusing is handled automatically.

No, updating the Zoom system beyond Windows 7 will render it inoperable.

We do allow windows updates to be installed on the controller; However, it will not be possible to update past feature pack 1607. The LTSB version supports installing security updates only.

We chose LTSB Version 1607 because we were able to validate it with the knowledge that Microsoft would not introduce new features on a regular basis.

The phase imaging and fluorescence excitation/emission wavelengths for each of the Incucyte^® models are available from your local Sales Representative or Applications Specialist.  Please contact your local representative for this information.

The list of morphological metrics available within your Incucyte^® software are dependent upon the software modules which have been purchased and installed.  Please contact your local Sales Representative or Applications Specialist for this information.

The most important considerations when choosing an incubator are the following physical parameters:

200 liter displacement

49 cm deep

The ability to maintain a stable environment, with 90% minimum Relative Humidity and 37 °C while in operation.

No. The Incucyte^® is a research device and is not intended to be used as a Medical Device. 

SX5/S3/SX1:

Imaging Specifications:

  20x:

      Image Resolution:  0.62 microns/pixel

      Image Size:  1408 x 1040 pixels

      Field of View:  0.88 x 0.65 mm

  10x:

      Image Resolution:  1.24 microns/pixel

      Image Size:  1408 x 1040 pixels

      Field of View:  1.75 x 1.29 mm

  4x:

      Image Resolution:  2.82 microns/pixel

      Image Size:  1536 x 1152 pixels

      Field of View:  4.34 x 3.25 mm

There are a few things you can try:

• Change the media. We don’t recommend leaving an open vessel in the Incucyte^® without a media change for more than a few days.
• Check the temperature on the status tab in the Incucyte^® software. It should be 37°C. If the temperature is too high, you may need to turn the temperature control of your incubator down to 36.5°C since the Incucyte^® does generate  heat during scans.
• Check the humidity in your incubator. We try to maintain Relative Humidity in the incubator at 95% RH (non-condensing).
• Fill the wells on the perimeter of a microplate tray with media only.
• Try sealing the plate with a breathable film.

Please power the system off completely via a shutdown through the front panel or GUI software: Device->Administer->Shutdown. After the system completely shuts down, you can open the front panel by pressing the side button and then press the top left black square power button to turn it back on, it should take 3-5 minutes to boot.