Range of Selectivity For Novel Modalities
New biotherapeutics such as nanobodies, bispecific mAbs and fusion proteins, present new challenges to traditional downstream purification platforms. Additionally, higher mAb titers and newly engineered mAb-like fusion proteins now drive the need for higher productivity/selectivity purification.
Sartorius Mixed-Mode (MM) chromatography ligands interact with target protein molecules through multiple types of interactions such as affinity, hydrophobic interaction (HIC), and cation/anion exchange. These multimodal separation mechanisms allow resins to be applied to solve challenging purification problems where traditional IEX, HIC, or affinity chromatography alone do not meet the need.
Matrices scalable from benchtop to commercial manufacturing
Unique mixed-mode ligands offer increased selectivity and resolution, offering a more powerful separation for complex purification
Plug-and-play chromatography matrices allow high-speed processing without sacrificing capacity
Novel biopharmaceutical targets such as nanobodies, multi-specific mAbs , and fusion proteins require new selectivities to achieve high product purity. Mixed-mode matrices leverage multimodal separation mechanisms such as IEX and HIC to achieve higher selectivity and purity in a single step compared to traditional single-mode chromatography.
Resins
Monoliths
Application
Capture and polish small and medium biomolecules
Capture and polish large biomolecules
Implementation
Multi-use
Intrabatch multi-use, single-use between batches
Ease-of-use
Bulk material (easy packing columns)
Ready-to-use
Scalability
Wide range of column sizes
Wide range of device sizes
Matrices
CMM, HEA, PPA, MEP, HA
H-bond ADC, PrimaS
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Novel biopharmaceutical targets such as nanobodies, plural-specific mAbs, and fusion proteins require unique selectivities to achieve high product purity. Mixed-mode resins leverage multi-modal separation mechanisms such as IEX and HIC to achieve higher selectivity and purity in a single step than in traditional single-mode chromatography.
Sartorius offers a broad range of selectivities to address challenges with a high concentration of contaminants with the intensified feedstreams, aggregates issues with newer modalities, and to remove challenging process or product-related impurities. Achieve reduced process costs by streamlining pre- or post-diafiltration steps.
CMM HyperCel
MEP HyperCel
HEA HyperCel
PPA HyperCel
HA Ultrogel®
Modes
CEX
HIC
Hydrophobic chargeinduction (HCIC)pseudo-affinityto IgG (thiophilic)
AEX
Pseudo-affinity
B/E antibodyHCP, aggregate removalrecombinant molecule purification
Capture of antibodies in physiological conditions (pseudo affinity) only for MEP HyperCel; HCP, aggregate, variants, removalantibody, fab, enzymes, recombinant proteins
Dynamic binding capacity
IgG>60-100 mg/mL*
Human IgG>20 mg/mL*
BSA40-60 mg/mL*
Cytochrome C>7 mg/mL**
Average particle size (µm)
50-80
80-100
60-180
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* 10% BT** 50% BT
CMM HyperCel is a hydrophobic cation exchanger recommended for the capture of antibodies, antibody fragments, and recombinant proteins. It allows the separation of proteins with similar pI by modulation of pH and conductivity.
MEP HyperCel provides unique selectivity for monoclonal antibody capture or purification to remove aggregates, HCP, and DNA. The ligand facilitates the binding of target proteins at neutral pH under moderate salt concentrations.
HEA HyperCel is a mixed-mode ligand with a less hydrophobic hexylamine chain allowing protein binding at a moderate salt concentration under neutral pH.
PPA HyperCel exhibits a stronger hydrophobicity with an aromatic ligand, allowing protein binding at a moderate salt concentration under neutral pH.
HA Ultrogel® hydroxyapatite is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed-mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. It is ideally suitable for general impurity removal.
The unique selectivity of mixed-mode monolithic columns ensures the proper purification of the most challenging large biomolecules.
CIMmultus® PrimaS
CIMmultus® H-Bond
Channel sizes
2 µm
1.3, 2 µm
Column volume
1 mL – 8 L
Working range, pH
2 – 13
Functionality
Hydrogen bonding and anion exchange
Multimodal hydrogen donor-acceptor
mRNA
Viruses and extra-cellular vesicles
Multimodal chromatography ligand that combines elements of hydrogen bonding with anion exchange chromatography
Unique selectivities in many important product areas
Supports purification of single-stranded RNA at ambient temperature
Fractionates ssRNA by size
Working range, pH 2–13
Enables size-based separation of product and contaminants at acidic pH
Elution with increasing salt, pH, or sorbitol
Highly tolerant of salt
Outstanding DNA removal
Unique selectivity for preparative and analytical applications
Sanitizable with 1 M NaOH
Learn how a batch process for mAb purification can be gradually transformed into an intensified process and then into a connected process.
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