CIM® Monolithic Chromatography Columns
Process Improvement With Monolithic Chromatography
CIM® (Convective Interaction Media) monoliths are designed as unique, single-unit structures – making them ideal for purifying very large biomolecules with a very small lab footprint. Large molecules are used commonly in modern gene therapy and vaccines products, and include virus particles, vesicles, RNA, plasmids, and other forms of DNA. These molecules require dedicated solutions for chromatographic purification.
The Sartorius family of CIM® monoliths is ideal for preparatory and analytical applications as well as immobilization screening. Pre-configured product packs are also available, a combination of columns and a protocol to suit specific needs.
Consult Our Experts
Large biomolecules are becoming increasingly popular in the biotherapeutic industry. Innovative solutions - such as monolithic chromatography - are needed to purify these new modalities.
Join us on a journey through the monolith matrix to discover how convective chromatography supports the robust separation of large biomolecules.
Join our webinar to learn what makes monoliths ideal for the challenges of purifying large biomolecules.
High resolution capabilities in combination with low shear flow properties for quality yields
Increased throughput from improved yields and faster run times, benefitting from high capacity and ready-to-use formats
Ready-to-use cGMP formats with proven scalability from 1 mL to 40 L
A robust tool for the purification of biomolecules in the nanometer range
IEX
HIC
Affinity
Mixed-Mode
CIMmultus® QA
CIMmultus® OH
CIMmultus® Oligo-dT
CIMmultus® PrimaS
CIMmultus® DEAE
CIMmultus® C4-HLD
CIMmultus® H-Bond
CIMmultus® EDA
CIMmultus® SO3
Choose Your Product
AAV & Other Viral Vectors
mRNA
pDNA
CIMmultus® Oligo dT
HiP2 Plasmid Process Pack
CIM® monoliths contain highly cross-linked, porous poly-methacrylate with well-defined channel size distribution. High surface accessibility of binding sites allows capacities beyond resin-based columns for large biomolecules, as well as rapid convection-based mass transfer.
With flow-independent performance, low back-pressure, and versatility our monolithic columns facilitate faster purification of your viruses or nucleic acids. CIM® monoliths are available in a variety of chemistries and sizes ranging from screening to commercial processing for higher yields and improved speed.
Provide information in real-time, through at-line monitoring of large biomolecules including viruses, pDNA & mRNA.
Sartorius experts represent extensive experience in chromatography and purification of biomolecules as well as analytical methods development.
Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers to meet the needs for purification of large biomolecules.
CIMmultus®DEAE
Channel sizes
1.3 | 2 | 6 µm
2 µm
Column volume
1 mL – 8 L
1 mL –8 L
Working range, pH
2 – 13
2 – 9
Functionality
Strong cation exchange
Strong anion exchange
Weak anion exchange
Typical application
Viruses, (i.e. AAV),and VLPs
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Strong cation exchanger
Working range, pH 2–13
Binds molecules with predominantly positive charge, repels molecules with predominantly negative charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for purification of virus and virus-like particles
Sanitizable with 1 M NaOH
Strong anion exchanger
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
High capacity for virus particles, IgM, exosomes
High-efficiency removal of endotoxin and DNA
Outstanding for preparative applications or analytical fingerprinting of process samples
Weak anion exchanger
Working range, pH 2–9
High capacity for plasmid DNA
Hydrophobic interaction chromatography (HIC) is a must for nucleic acid separations. When combined with the advantages of monolithic chromatography, HIC meets this need while also providing an excellent solution for the purification of large biomolecules including adenoassociated viruses (AAV).
1.3, 2, 6 µm
2, 6 µm
2 – 10
Weak hydrophobic
Strong hydrophobic
Viruses, (i.e. AAV), and VLPs
Nucleic acids (pDNA, mRNA)
Uncharged (neutral) ligand
Working range, pH 2–10
Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol
Elutes large solutes with high resolution in order of increasing size
Elution with descending salt or polyethylene glycol
Strongly hydrophobic, (uncharged) ligand
Highly effective for removing proteins from nucleic acids (pDNA, RNA)
Monolithic columns combined with unique affinity ligands offer an unmatched solution for affinity purification of large biomolecules.
Oligo dT ligands covalently bound to solid support
Hybridizes to poly-adenylated tail found on most eukaryotic mRNAs, or synthetized onto the molecule during IVT
Fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
Selectively removes impurities from samples
Convenient approach for initial purification of mRNA
Available with:
The unique selectivity of mixed-mode monolithic columns ensures the proper purification of the most challenging large biomolecules.
1.3, 2 µm
Hydrogen bonding and anion exchange
Multimodal hydrogen donor-acceptor
Application
Viruses and extra-cellular vesicles
Multimodal chromatography ligand that combines elements of hydrogen bonding with anion exchange chromatography
Unique selectivities in many important product areas
Supports purification of single-stranded RNA at ambient temperature
Fractionates ssRNA by size
Enables size-based separation of product and contaminants at acidic pH
Elution with increasing salt, pH, or sorbitol
Highly tolerant of salt
Outstanding DNA removal
Unique selectivity for preparative and analytical applications
Process Packs support the development and scale-up of large molecule purification. They come with recommended CIMmultus® columns and detailed process instructions to achieve high-quality purification in a short time.
HiP2 Plasmid Process Pack™
1 – 80 mL
Included chemistries
Additional components
Detailed process instructions
HiP² Plasmid Process Pack™ is a ready-to-use, adjustable pDNA purification pack, to help develop your own pDNA purification process and transfer it to production.
Detailed experiments are included, saving save time and money.
The process is extremely flexible, and allows choosing only parts of the protocol that fit your needs. CIMmultus® HiP² Plasmid Process Pack™ contains one weak anion-exchange (DEAE) and one hydrophobic (C4 HLD) column and a purification protocol of your plasmid.
Validate the viral reduction performance of Virosart® HF with our Confidence® Services: Highly purified viruses and expert knowledge.
This webinar features an exclusive panel discussion where the speakers lay out their vision for truly optimized next-gen mRNA processes.
Learn how a new, optimized purification approach for mRNA provides higher yields and lower costs
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