Octet® R2 Protein Analysis System
Octet® R2 Protein Analysis System: Grows With Your Biomolecule Characterization Needs
The Octet® R2 System makes high-quality interaction analysis affordable and flexible. Priced for startup academic and biotech labs and low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The 2-channel Octet® R2 system offers an advanced, fluidics-free approach to interaction analysis, with a wide variety of off-the-shelf Dip and Read biosensors for binding kinetics and quantitation analysis. Octet® R2 provides a low-cost entry point for labs with lower throughput needs, while providing the flexibility to field-upgrade to 4 or 8 channels anytime the need arises.
The Octet® R2 System characterizes protein-protein and protein-small molecule binding interactions for early research and discovery. The multipurpose system also provides quantitative information by measuring active protein concentrations — even in complex mixtures like cell culture supernatants and lysates. With sensitivity for small molecule analysis, the Octet® R2 instrument enables the measurement of interactions between small molecules down to 150 Da in size, and their larger binding partners to determine accurate kinetics rates.
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The Octet® R2 System monitors binding events in real- time measuring binding association (ka) and dissociation (kd) kinetics, and affinity constants (KD). The system’s two channels can be used independently for screening purposes, or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.
Samples are loaded in standard microplates, eliminating fluidic line maintenance and expanding the number of samples that can be run in an assay. Pre-defined templates in Octet® BLI Discovery software streamline setup prior to running an assay and minimizes training needs. The software also offers users a range of parameters and metrics for analyzing acquired data in a powerful yet intuitive software interface.
The 2-channel Octet® R2 system directly detects the presence of specific proteins in solution with minimal interference from complex matrices. Accurate and reproducible concentrations can be determined in as little as 2 minutes per sample using a simple, one-step assay.
The sample plate temperature can be controlled from 15–40°C, which enables reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic parameters.
The Octet® R2 system provides the ability to re-rack biosensors at the end of an experiment. Biosensor re-racking provides enhanced flexibility in loading ligands on biosensors and reduces operational costs when biosensors are loaded with precious target reagents.
Robust technology with simple, integrated user interface designed for biologists. Spend less time learning instrumentation and more time deriving insights.
With pre-defined templates to follow in the Octet® BLI Discovery software, the Octet® R2 system streamlines setup prior to running an assay and minimizes training needs. Octet® Analysis Studio then gives users a range of parameters and metrics for analyzing acquired data, in a powerful yet intuitive software interface.
With sensitivity to screen small molecule fragment libraries, the Octet® R2 instrument enables the measurement of interactions between small molecules down to 150 Da in size to determine accurate kinetics rates.
Read Application Note
Literature references covering a large variety of research areas and applications
Accelerate your research with an upgradeable, label-free protein analysis platform
Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment
Tips collected over the years from customers successful with their equipment grant proposals.
A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...
Developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032
Using Octet® BLI Technology
Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.
Learn how Octet BLI transforms our approach to drug discovery and development
Bio-Layer Interferometry (BLI) based on fiber optic biosensors
Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the biosensor tray
Data Presentation
Sample Type
Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and virus-like particles in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants and crude cell lysates
Number of Spectrometers
2
Maximum Simultaneous Reads
Data Collection Rate
2, 5 or 10 Hz
Sample Position and Format
1 standard 96-well, black, flat-bottom microplate
Sample Volume
Orbital Flow Capacity
Static or 400–1500 rpm
Analysis Temperature Range
15°C to 40°C, in 1°C increments
Quantitation and Kinetics
Throughput
Up to 2 assays in parallel
Molecular Weight Detection
>150 Da
Analysis Time per Sample
10-6 to 10-1 s-1
1 mM – 10 pM
Quantitation Range
0.5 µg/mL to 2000 µg/mL (Human IgG and Protein A Biosensor) Protein - and assay-dependent
Baseline Noise (RMS)
<3.0 pm
Baseline Drift
< 0.1 nm/hour
Quantitation Precision
CV <10%
Instrument
Weight
72 lb (32.7 Kg)
Dimensions (H x W x D)
The perfect balance between cost and throughput with given leeway
Unmatched flexibility and versatility in protein analysis
Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements
Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed
Affordably priced label-free analysis for individual researchers on any budget
The Octet® AS enables simultaneous and uniform reagent loading onto all 96 biosensors in a biosensor tray.
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