Octet® R4 Protein Analysis System
The 4-channel Octet® R4 instrument can be used for a wide range of analyses including kinetic analysis, titer of IgG and other proteins, reagent qualification, immunoassay development, bioprocess development, quality analysis, crude antibody screening, epitope binning/mapping, ligand binding assays, and small molecule analysis, elucidating cell signaling mechanisms, and infectious disease monitoring. Analysis can be done using a single to all four channels, enabling flexibility in sample throughput.
The Octet® R4 System is priced for customers looking for a balance between throughput and cost. This system provides higher throughput than the 2-channel Octet® R2 instrument, and the same sensitivity as the 8-channel Octet® R8 instrument - at a lower overall price. Contact us and we’ll help determine which throughput level is best for your lab and research needs.
Request an Octet® R4 Quote or Demo
Boost your research and commercialization goals by combining BLI and SPR at an attractive promotional price.
The Octet® R4 system has four parallel, independent channels for increased flexibility and faster results.
The Octet® R4 system is designed for customers looking for moderate throughput sample analysis, without any compromise on assay sensitivity.
The Octet® platform provides faster time to results and process economy when compared to other technologies due to its fluidics-free Dip and Read format. Additionally, crude sample compatibility saves valuable time by eliminating the need for sample pre-treatment, and non-destructive testing conserves precious samples for other assays.
Choose when to upgrade to an 8-channel Octet® system for higher throughput and full GMP compliance. Upgrades are performed on-site within a single visit by trained Sartorius engineers without the hassle of down time or system trade-in.
The sample plate temperature can be controlled from 15–40°C, which enables reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic measurements. The Octet® R4 system’s four channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.
The Octet® R4 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and affinity constants (KD) by bringing the detection surface directly to the sample, eliminating the need for microfluidics.
The sample plate temperature can be controlled from 15–40°C, which enables reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic measurements.
Accurately determining the concentration of antibodies and other proteins is critical to the selection of cell lines for development and the optimization of antibody production.
The Octet® R4 system directly measures the presence of specific proteins and other molecules in solution with minimal interference from complex matrices with reproducible concentrations determined in as little as 2 minutes per sample using a simple, one-step assay. Rapid turnaround time of results improves process efficiencies and productivity that cannot be achieved with othermethods such as HPLC and ELISA.
Literature references covering a large variety of research areas and applications
Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment
Accelerate your research with an upgradeable, label-free protein analysis platform
A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...
Learn how Octet BLI transforms our approach to drug discovery and development
Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.
Using Octet® BLI Technology
Developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032
Bio-Layer Interferometry (BLI)
Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the biosensor tray
Data Presentation
Sample Type
Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and VLPs in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
Number of Spectrometers
4
Maximum Simultaneous Reads
Data Collection Rate
2, 5 or 10 Hz
Sample Position and Format
1 standard 96-well, black, flat-bottom microplate
Sample Volume
Orbital Flow Capacity
Static or 100–1500 rpm
Analysis Temperature Range
15°C to 40°C, in 1°C increments
Quantitation and Kinetics
Throughput
Up to 4 assays in parallel
Molecular Weight Detection
>150 Da
Analysis Time per Sample
Real-time kinetic binding experiments from 5 minutes to 4 hours
1 mM – 10 pM
Baseline Noise (RMS)
<3.0 pm
Baseline Drift
≤ 0.12 nm/hour
Quantitation Precision
CV <10%
Instrument
Weight
72 lb (32.7 Kg)
Dimensions (H x W x D)
Grows with your biomolecule characterization needs
Unmatched flexibility and versatility in protein analysis
Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements
Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed
Affordably priced label-free analysis for individual researchers on any budget
The Octet® AS enables simultaneous and uniform reagent loading onto all 96 biosensors in a biosensor tray.
Get the Latest Updates, Trends and Developments on Label-free, Stress-Free Solutions for Drug Discovery, Biologics Development and Biomanufacturing.
We're offering an incredible deal - Bundle an Octet® BLI and SPR instrument and benefit from exceptional savings! This is your chance to bring the top two technologies for label-free biomolecular interaction analysis to your lab.
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