Octet® R8 Protein Analysis System
The Octet® R8 label-free protein analysis system provides fast, high throughput, and accurate characterization of biomolecules and is compatible with longer experimental run times and seamless integration into GxP-compliant labs during biologics development.
The 8-channel Octet® R8 system performs quantitation and kinetic analysis of up to 96 samples in 30 minutes to 2.5 hours, depending on the specific assay. An optional microplate evaporation cover minimizes losses in sample volume, allowing post-analysis sample recovery even after an experiment.
The Octet® R8 system can be used for a wide range of analyses including kinetic analysis, titer of IgG and other proteins, reagent qualification, immunoassay development, bioprocess development, quality analysis, crude antibody screening, epitope binning/mapping, ligand binding assays, small molecule analysis, elucidating cell signaling mechanisms, and infectious disease monitoring. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput.
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The Octet® R8 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and affinity constants (KD).
The high sensitivity Octet® R8 system detects a diverse range of biomolecules from small molecules to proteins to cells.
The Octet® R8 system’s eight channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.
Optional 21 CFR Part 11 software for Octet® R8 system available along with installation and qualification (IQOQ) and performance qualification (PQ) kits.
The sample plate temperature can be controlled from 15–40°C, enabling reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic measurements. The Octet® R8 system’s eight channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.
The Octet® platform provides faster time to results and process economy when compared to other technologies due to its fluidics-free design. Additionally, crude sample compatibility saves valuable time by eliminating the need for sample pre-treatment, and non-destructive testing conserves precious samples for other assays.
The superior sensitivity of the Octet® R8 system enables measurement of both small and large molecules and kinetic constants over a broad range. The system accurately measure kinetic constants by bringing the detection surface directly to the sample, eliminating the need for microfluidics.
With ready to use biosensors for most proteins, the unique Dip and Read approach streamlines workflows and expedites assay development. It allows for direct measurement of crude samples with minimal instrument maintenance.
Accurate and reproducible concentrations can be determined in as little as 2 minutes per sample or 32 minutes for a whole 96-well plate using a simple, one-step assay.
High sensitivity in quantitation can be easily achieved by increasing read time and shake speed, and even higher sensitivity can be achieved to sub-ng/mL levels with 2-step or 3-step assay formats, allowing automated measurement of contaminants such as host cell proteins and residual protein A, faster and more precisely than ELISA. Process economics can be improved further by regenerating and reusing the biosensors.
The Octet® R8 system has been developed to operate reliably in regulated quality control (QC) environments. The Octet® is aptly used in method development and validation for lot release and in-process testing for ligand binding, concentration, and impurity analysis in both upstream and downstream processes.
The ease of use and simplicity in design together with 21 CFR Part 11 software and a full line of GxP products and services makes the Octet® R8 system an ideal analytical instrument for compliant labs.
Read Application Note
Literature references covering a large variety of research areas and applications
Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment
Octet® GxP packages support the GMP compliant implementation, including 21 CFR Part 11 software, validation packages, and biosensor validation support...
Tips collected over the years from customers successful with their equipment grant proposals.
Accelerate your research with an upgradeable, label-free protein analysis platform
Developing Methods for Comparability Studies of Therapeutic Monoclonal Antibodies
A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...
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Using Octet® BLI Technology
Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.
Bio-Layer Interferometry (BLI)
Data Presentation
Sample Type
Number of Spectrometers
8
Maximum Simultaneous Reads
Data Collection Rate
2, 5 or 10 Hz
Sample Position and Format
1 standard 96-well, black, flat-bottom microplate
Sample Volume
Orbital Flow Capacity
Static or 100–1500 rpm
Analysis Temperature Range
15°C to 40°C, in 1°C increments
Quantitation and Kinetics
Throughput
Up to 8 assays in parallel
Molecular Weight Detection
>150 Da
Analysis Time per Sample
10-6 to 10-1 s-1
1 mM – 10 pM
Baseline Noise (RMS)
<3.0 pm
Baseline Drift
< 0.12 nm/hour
Quantitation
Workflow
Human IgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples
DirectQquantitation Range for Human IgG with Protein A Biosensor
0.05 –2000 μg/mL
Instrument
Weight
Dimensions (H x W x D)
Grows with your biomolecule characterization needs
The perfect balance between cost and throughput with given leeway
Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements
Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed
Affordably priced label-free analysis for individual researchers on any budget
The Octet® AS enables simultaneous and uniform reagent loading onto all 96 biosensors in a biosensor tray.
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