96-Channel Ultra High Throughput Octet® RH96
From IgG titer to epitope binning, the Octet® RH96 instrument offers label-free molecular interaction analysts with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.
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The Octet® RH96 instrument read head provides design flexibility that allows users to analyze, 8, 16, 32, 48, or 96 samples at a time allowing for customized throughput up to 96 samples.
The Dip and Read assay format allows off-rate analysis of clones to be performed rapidly, even in crude samples using ready-to-use biosensors off the shelf.
IgG titer determination of a full 96-well plate can be completed in two minutes. In addition, sample plate compartment is compatible with 384-well plates allowing for greater sample throughput.
With the Octet® RH96 system, epitope binning can be completed in record time, where a 32 x 32 antibody matrix can be analyzed in less than eight hours. A powerful dedicated epitope binning analysis software enables easy visualization and analysis of large data sets.
The Octet® RH96 system utilizes Dip and Read technology where biosensors dip into samples housed in microplates. With no fluidics involved, Octet® systems have more uptime and lower operational costs
ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet® systems are designed to overcome those limitations. Convert your ELISA assay into an Octet® assay.
Read Application Note
The epitope binning process identifies and arranges antibody candidates that bind similar epitope regions to an antigen into bins. Octet® epitope binning assays rely on testing the sequential binding ability of two antibodies to an antigen. Assay speed, throughput, exceptional reproducibility, and dedicated software tools make Octet® platforms ideal for analyzing large antibody libraries.
Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits provide biologics developers with a fast and easy-to-use assay for contaminant testing.
Download Technical Note
Epitope binning through cross competition assays is a powerful tool in evaluating functional diversity in antibody clones.
A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...
Improve the overall drug discovery process to increase the success rate of preclinical candidates
Octet® GxP packages support the GMP compliant implementation, including 21 CFR Part 11 software, validation packages, and biosensor validation support...
Technologies, Key Considerations, and Applications
Developing Methods for Comparability Studies of Therapeutic Monoclonal Antibodies
Scientists from Elanco® use Octet® BLI to rapidly design and establish a preclinical PK method for analyzing canine antibodies
Technology
Bio-Layer Interferometry (BLI) based on fiber optic biosensors
Sample volume
40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered
Data presentation
Sensorgrams displaying kinetic traces or concentration binding rates Epitope binning and cross-blocking matrices and trace overlays Tabulated kinetic or concentration data
Automation
Robot compatible microplate and biosensor tray loading
Orbital flow capacity
Static or 100–1500 rpm
Analysis temperature
Ambient + 4°C to 40°C, in 1°C increments
Association rate constant (ka)
101 to 107 M-1 s-1
Dissociation rate constant (kd)
10-6 to 10-1 s-1
Affinity constant (KD)
1 mM – 10 pM
Baseline noise (RMS)
<3 pm (8–16 biosensors); <8 pm (32–96 biosensors)
Baseline drift
< 0.1 nm/hour
Quantitation range
32–96 biosensors: 0.1–100 μg/mL of hIgG at 1000 rpm**; 1.0–700 μg/mL at 400 rpm 8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm
Quantitation precision
CV <10%
Weight
200 lbs (90.7 kg)
Dimensions (H x W x D)
30.1 in x 31.5 in x 31.5 in (77 cm x 80 cm x 80 cm)
Sample type
Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates
Sample plate
Standard, 96-well and 384-well, black, tilted bottom microplates
Molecular weight detection
>150 Da (8–16 biosensors), >5000 Da (32–96 biosensors)
Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements
Unmatched flexibility and versatility in protein analysis
The perfect balance between cost and throughput with given leeway
Grows with your biomolecule characterization needs
Affordably priced label-free analysis for individual researchers on any budget
The Octet® AS enables simultaneous and uniform reagent loading onto all 96 biosensors in a biosensor tray.
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