TFF Systems For Ultrafiltration and Diafiltration | Sartorius

TFF Systems - Process Solutions for Ultrafiltration and Diafiltration

Sartorius offers flexible tangential flow filtration (TFF) systems for process development and clinical trials, from laboratory environments up to commercial production batches. The TFF portfolio can flex to accommodate individual requirements, with a range of options that includes cost-efficient multi-use systems or closed systems with single-use flow kits for aseptic processing.


 

Find the Right TFF System

A range of TFF systems and components are available, offering flexibility and performance, from process development to commercial-scale in GMP environments.

Attributes

Ambr® Crossflow

Sartoflow® Smart

Sartoflow® Advanced

Sartoflow® 150

Membrane Area

10, 50 cm²

50 cm² - 0.14 m²

0.1 – 0.21 m²

0.1 – 0.4  m²

Vessel Volume

100 mL

1 L with mixing

10 L

5, 10, 20 L

Flow Rate / Pump Capacity

0 – 80 mL/min

1 – 100 L/h

700 L/h

5 – 150 L/h

Minimum Recirculation Volume

5 mL

< 20 mL

~ 170 mL

~ 130 mL

Automation Level

Fully

Semi

Fully

Fully

Process Mode (multi/single)

Multi-use or single-use

Multi-use

Multi-use

Single-use

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Attributes

Sartoflow® Expert SU

Sartoflow® 1000

Sartoflow® 4500

Membrane Area

Flat sheet: 0.1 – 0.4, 1.4 m²

Flat sheet: 0.6 - 2.8 m²
Hollow fiber: 0.13 - 2.5 m²
Flat sheet: up to 10 m²
Hollow fiber: 2.69, 5 m²

Vessel Volume

20 L mixing

10, 20, 100 L

 

Flow Rate / Pump Capacity

900 L/h

20 – 900 L/h

100 – 4500 L/h

Minimum Recirculation Volume

~ 600 mL

~ 1.2 L

Depends on bag size

Automation Level

Fully

Fully

Fully

Process Mode (multi/single)

Single-use

Single-use

Single-use

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PD & Bench Scale TFF Systems

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Ambr® Crossflow — High-Throughput for Parallel Screening

Ambr® Crossflow provides molecule behavior data in early development on viscosity, buffer composition, shear stress and performance in membrane processes. It also contributes important additional decision criteria to help guide and accelerate the candidate selection process.

Ambr® Crossflow allows developers to study factors relevant to manufacturability, providing the capacity for up to 16 automated, parallel trials. The system is capable of processing volumes as low as 5 mL recirculation volume. 

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Sartoflow® Smart — The Ideal TFF System for Process Development

The Sartoflow® Smart is a modular and flexible small-scale benchtop TFF system for ultrafiltration and diafiltration. It is designed for laboratory environments and suitable for process development, clinical trials and small batch cGMP production. Sartoflow® Smart is used in many downstream processes, such as purification of vaccines, monoclonal antibodies and recombinant proteins.

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Sartoflow® Advanced — Full Flexibility Meets Outstanding TFF Performance 

The Sartoflow® Advanced is a modular benchtop TFF system with a variety of options for ultrafiltration and diafiltration.

  • Designed for both process development and small-scale production 
  • Accepts either Sartocon® Slice or full-sized Sartocon® cassettes for a range of membrane surface areas
  • Recirculation tank or single-use bags
  • User-friendly touch screen control interface

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Sartoflow® 150 – Efficient Single-Use TFF Processing up to Preclinical Scale

The Sartoflow® 150 is an automated single-use tangential flow filtration system (SUTFF). Its compact footprint and low recirculation volume are optimized for small-scale process ultrafiltration and diafiltration (UF/DF) applications of 2–20 L batch volumes.

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Connected and Continuous Ultrafiltration and Diafiltration

SPTFF simplifies ultrafiltration and diafiltration with an inflow process mode. It helps mAb manufacturers shorten setup and enhance efficiency.

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Pilot to Commercial Scale TFF Systems

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Sartoflow® Expert SU — Versatile TFF and Safe ADC Synthesis 

Sartoflow® Expert SU is a fully automated TFF system with a closed single-use loop for PD up to pilot. In addition to state-of-the-art TFF, it can be configured to streamline 3 unit operations for 1-20 L ADC synthesis on one system, thus increasing safety and quality. 

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Sartoflow® 1000 — Process Control and Ease of Use at Pilot Scale

The Sartoflow® 1000 facilitates automated processing of monoclonal antibodies, recombinant proteins and vaccines in batch volumes from 20 to 200 L.

An intuitive interface design simplifies automation of TFF process sequences, with complete single-use flow kits that incorporate all critical sensor and instrumentation technologies.

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Sartoflow® 4500 - Process Control and Ease of Use at Commercial Scale

The Sartoflow® 4500 single-use tangential flow filtration (SUTFF) system has a user-friendly control interface for operating fully automated ultrafiltration and diafiltration (UF/DF) applications for batch volumes of 200 to 2000 L (1 to 15 kg). The Sartoflow® 4500 is compact, has low hold-up volume, and the capacity to run cassette surface areas up to 10 m².

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Related Products

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TFF Cassettes

Hydrosart® TFF cassettes let you focus less on cleaning and sterilizing due to exclusive membrane tech with high chemical resistance and long product...

TFF Holders

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