Transfection Reagents and pDNA Solutions
Transient transfection is a method of choice to modify the expression of specific gene(s) in eukaryotic cells. It requires three main components to create a transfection mix which are the nucleic acids (pDNA, mRNA, siRNA, etc), the transfection reagents and a complexation buffers/media.
The delivery system needs to be carefully selected based on the application and its challenges:
Choose among a broad range of powerful transfection reagents designed for specific application to be successful in your projects. Transfection reagents are ready-to-use with a pre-optimized protocol to ensure high transfection efficiency while preserving cell viability and morphology.
We assist academics, biotech and Pharma companies from R&D to manufacturing with end-to-end nucleic acid services, covering plasmid design, plasmid engineering, plasmid manufacturing (research to GMP), plasmid sequencing and process development and manufacturing of new biologics (viral vectors, recombinant proteins, antibody fragments, biosimilars and biobetters).
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Available at research and GMP grade to intensify production of recombinant AAV.
A leading PEI-based DNA transfection reagent that offers flexibility and scalability in viral vector manufacturing process.
Transfection reagent designed to improve Lentiviral vector (LV) titers in HEK-293 suspension cell systems.
A novel plasmid helper designed for AAV production.
Ready-to-use RepCap plasmid for AAV2 production.
Take a look at our innovation solution that offers gold-standard products and services from research to commercialization that enhance the quality and reliability of:
The development of robust, scalable, cost-effective processes that yield high titers of high-quality viral vectors is crucial to the successful commercialization of safe and efficacious gene therapies that require large and/or multiple doses to treat more prevalent diseases. The successful commercialization of viral vector-based therapies relies on GMP-compliant and scalable, industrialized production platforms to rapidly advance high-quality products to market while also ensuring patient safety.
The most utilized approach to AAV (adeno-associated virus) and lentivirus (LV) production is through the transient transfection of mammalian cell lines, such as HEK293 or derivatives, followed by viral vector harvest, downstream purification processes and analytics for final product testing.
Since viral vector production is a significant cost driver in AAV and LV-based gene therapies, it is prudent to optimize each step of the upstream manufacturing process already at the process development phase to ensure compliance of critical raw materials (eg. plasmid DNA, transfection reagents), maximize yield and product quality while minimizing costs.
FectoVIR®-LV transfection reagent designed to improve Lentiviral vector (LV) titers in HEK-293 suspension cell systems.
A novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in suspension.
A set of ready-to-use RepCap plasmids for AAV production of various serotypes through triple transfection. Optimized for use with pPLUS® AAV-Helper.
Optimize your process and prepare for scale up with fewer trials.
Non-viral in vivo delivery reagents are the most powerful alternative to viral vectors for nucleic acid delivery. They offer substantial advantages in terms of reliability, safety and costs for nucleic-acid based therapies. Different quality grades of in vivo transfection reagents are available for proof concept studies, preclinical studies and clinical trials.
A novel range of proprietary cationic lipids dedicated to the formulation of LNPs for the development of mRNA-based vaccines and therapeutics.
Ready-to-use transfection reagent composed of lipid-based nanoparticles specifically developed for both in vivo and ex vivo mRNA delivery.
Ready-to-use cationic polymer reagent recommended for in vivo transfection of DNA, siRNA, miRNA, shRNA and other oligonucleotides.
Transient production of recombinant protein or monoclonal antibodies is an important step in research to study the behavior of a protein of interest in a cell, to screen and candidate proteins in early stages of therapeutic research and to enable modulation of molecular mechanism in many other fields of application.
Our goal is to help you make this protein production step as seamless as possible by optimizing the genetic engineering of your plasmid of interest and the transient expression system, whether bacterial, yeast or mammalian cell-based to meet your requirements for protein yield, function and quality.
What we can provide:
FectoPRO® is a transfection reagent specifically optimized for transient protein and antibody expression in CHO and HEK293 cells.
FectoCHO® expression system is optimized for culture and transient protein expression of all CHO cells.
Explore our extensive range of life science delivery solutions for scientists, including ready-to-use transfection reagents suitable for most mammalian primary cells and cell lines, as well as off-the-shelf plasmids. These products ensure that you can conduct your research smoothly and rely on the following approaches:
A versatile DNA and siRNA transfection reagent that ensures high DNA transfection efficiency and excellent gene silencing.
Leverages innovative cationic nanotechnology developed to improve DNA transfection efficiency in hard-to-transfect cells.
Achieves over 90% gene silencing in a wide variety of cells; suitable for transfecting miRNA and other oligonucleotides.
An efficient and gentle mRNA transfection reagent for high mRNA transfection in primary cells, cancer cell lines, neurons, and stem cells.
A robust and reproducible transfection reagent, ideal for automated or manual high-throughput screening workflows.
A powerful and efficient reagent for delivering proteins, antibodies, and peptides into mammalian cells.
Our pDNA services helps you to have as much control as you want over the plasmids you use. Allowing you to progress with confidence in your research and ensure the level of performance you need, whether it be tissue-specific expression, high yield protein expression, RNA synthesis or high viral titers for therapeutic purposes.
We offer tailor-made plasmids with fully flexible content, designed by you and our plasmid scientists, ready-to-use and manufactured at any scale and at any quality grade from research to GMP grade
Tailor-made DNA plasmid that really fits your needs from discovery grade to GMP grade manufacturing.
Plasmid DNA manufacturing using an unique process for Research, HQ and GMP grades
Full-lenght plasmids sequencing to provides high-quality and accurate sequencing of any plasmid.
Discover our range of easy & ready-to-use plasmids. Designed to cater to your specific needs by ensuring a seamless and cost-effective experience.
Our online plasmid design platform offers a unique intuitive interface to modularly create any kind of plasmid, to fit your experimental needs. This online portal is also the entry-point to our plasmid building platform operated with the e-Zyvec® DNA assembly technology. This allows us to robustly assemble the desired plasmids, regardless of sequence complexity. All our products can also be up-scaled from discovery grade, to R&D, to HQ and to GMP grades. Our pDNA Services:
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From starting transfection, changing plasmid DNA construct, working with a new cell line or isolated primary cells can require fine-tuning. Our transfection experts are always available to help you.Contact Support Team
Scientists rely on transfection as a powerful technique to modulate gene expression in eukaryotic cells in vitro and in vivo. Transfection can be used from researchers in universities or research institutes to engineers in Biotechnology or Pharmaceutical companies.
Transfection is the process that allows exogenous nucleic acids to bypass the cell membrane to enter into cells. Exogenous nucleic acids commonly used are plasmid DNA, RNA, siRNA and oligonucleotides. Once delivered into cells, nucleic acids modulate gene expression by driving overexpression or silencing of a gene of interest.
Gene overexpression is an indispensable tool for several applications, from understanding the role of gene of interest (gene studies, high-throughput screening), to the production of biologics such as antibodies (protein production) and recombinant viral particles, particularly for therapeutic purposes (virus production for gene & cell therapy).
Gene silencing is a method used to prevent expression of a gene of interest. The expression of a gene can be partially reduced (gene knockdown) or completely blocked (gene knockout). Because any gene can potentially be targeted, gene silencing is a prevalent technique used to develop gene-based therapies to address monogenic pathologies, cancer and in immunotherapy strategies.
Transfection of nucleic acids is used to transiently or stably modifed cells by overexpressing or silencing specific gene(s). Several methods can be used to performed transfection that are generally divided in two different categories: Chemical and physical. There are several physical methods that exist such as electroporation, sonoporation or microinjection but these processes are complex and relatively toxic for mammalian cells. To solve these issues, chemical-mediated transfection offers a great alternative: easiness of use, high transfection efficiency and excellent cell viability. Chemical transfection are typically performed using cationic polymers or lipids that will protect the anionic nucleic acids.
Novel engineered pHelper plasmid to improve yield and quality of several AAV serotypes in suspension cell culture systems.
Next-generation transfection reagent for large scale AAV manufacturing.
Cell and gene therapy solutions driven by reproducibility, scalability, and support for regulatory compliance.
Improve process economics in cell and gene therapies.
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