On-Demand Webinar: Accelerate Assay Development and Reduce Cycle Times for AAV Quantification in Complex Biological Samples

The assessment of titers, integrity, and other critical quality attributes (CQAs) of AAV vectors has become a pressing issue in gene therapy manufacturing. In the process of developing their own AAV platform, ExcellGene faced the need for a potent and robust method for the assessment of titers and quality of AAV viral particles present at different steps in the manufacturing process. However, the use of serotype-specific ELISA as the current gold standard method is not only time consuming, with a turnaround time of four to five hours, but also prone to significant matrix effects.

The use of a label-free approach not only helped in reducing these effects in complex biological matrices, but also allowed ExcellGene to set up a routine method easily and rapidly for the high-throughput and automated analysis of up to 96 samples in less than 30 minutes.

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Yoann Saucereau, Ph.D.

Research Associate Analytics at ExcellGene

Yoann Saucereau obtained his PhD in microorganism interactions and infection at the Claude Bernard University in Lyon in 2016, where he explored the molecular and immune mechanisms of microbial interference in mosquitoes before completing a post-doc at the Department of Biochemistry at the University of Cambridge, UK, where he continued to study this topic using biophysical approaches to characterize these interactions. With this experience in studying interaction models and using state-of-the-art techniques such as BLI and SPR, he joined Excellgene as an Analytical Research Associate to bring his experience to integrate and develop new innovative analytical methods.