Early identification of antibody candidates with desired affinities and dissociation kinetics, binding epitopes, and critical quality attributes such as glycosylation profiles, among others, can be vital for avoiding later-phase failures caused by selecting non-ideal leads. The epitope binning process enables antibody candidates that bind similar epitope regions in the antigen into bins.
Fluidic-free biolayer interferometry (BLI) enables epitope binning analysis using cross-competition assays where competitive binding of antibody pairs are assessed. These assays can be easily extended to characterizing large antibody libraries utilizing high-throughput Octet® BLI platforms and specifically developed epitope binning software analysis tools to evaluate large antibody data sets. Octet® epitope binning assays can also accommodate multiple assay orientations that enable analysis of multivalent antigens and antibodies in crude or hybridoma supernatants.
This application note outlines the capabilities of advanced biolayer interferometry systems and provides guidance on developing epitope binning assays and data analysis strategies.
Get the Latest Updates, Trends and Developments on Label-free, Stress-Free Solutions for Drug Discovery, Biologics Development and Biomanufacturing.
Please select your country so we can show you products that are available for you.
The content of our website is always available in English and partly in other languages. Choose your preferred language and we will show you the content in that language, if available.
Shop Sartorius Products Now