Optimization of Human iPSC-derived Neuronal Mono- and Co-cultures with Microglia

Authors: Jasmine Trigg, Daryl Cole, Natasha Lewis, John Rauch, and Nicola Bevan

Models derived from human induced pluripotent stem cells (iPSCs) are becoming more prevalent for in vitro research on the human central nervous system. These models facilitate the creation of specific types of neurons and supporting cells, offering a system that more closely mirrors human biology for the study of development and disease.  

The role of growth factors and cytokines is crucial in these models for promoting cell growth, differentiation, and survival. However, their analysis is often complex due to variables such as undisclosed ingredients in commercial media and the cells' sensitivity to manipulation.

To effectively utilize these sophisticated models, it is essential to establish stable culture conditions and employ technological methods that allow for the non-destructive optimization, visualization, and dynamic measurement of these intricate systems.