Phagocytosis | Sartorius

Measure Phagocytosis in Real Time

The Incucyte® Live-Cell Analysis System enables real-time, automated phagocytosis assays within your cell culture incubator.


  • Visualize and quantify phagocytosis using time-lapse imaging
  • Automated analysis over the entire assay time course
  • Simple mix and read 96/384-well protocols, no washing, no fixing, no lifting
  • Highly sensitive measurements, low background and low cell numbers


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Measuring Phagocytosis of Cells with Incucyte® Phagocytosis Assay

  • Automatically quantify the phagocytosis of cells in real time
  • Investigate efferocytosis and antibody mediated cellular phagocytosis (e.g., anti-CD47 / ADCP)
  • Compatible with your choice of target cells labeled with the pHrodo® Cell Labeling Kit for Incucyte® 


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Measuring Phagocytosis of Bioparticles® with Incucyte® Phagocytosis Assay

  • Monitor and measure uptake of bacteria or yeast pHrodo® Bioparticles® for Incucyte®
  • Validate phagocytosis with images and movies
  • Study the effect of treatments on full time course phagocytosis profiles 


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Incucyte® Phagocytosis Assays vs. Other Common Approaches

Common methods used to assess phagocytosis are often single point (e.g. High Content Analysis (HCA)), require cell lifting (e.g. flow cytometry) or washing/quenching of the labelled pathogen (e.g. fluorescein labelled).


Incucyte®    

Plate reader    

Flow cytometry    

HCA

ELISA

Real-time cell visualization    

 

Integrated analysis    

 

 

 

Mix-and-read, no wash or quench    

 

Long-term kinetic measurements    

 

Flexible choice of effector cells    

 

 

 

 

 

No fixation/lifting    

 

 

High sensitivity/low background    

 

 

 

Low cell number/bioparticle number    

 

 

 


Literature and Documentation

eBook

Live-Cell Analysis Handbook: 6th Edition

Discover how the power of artificial intelligence for label-free, live-cell imaging and analysis is moving research forward and driving deeper discove...

cytokine app note

Application Note

Gain additional insights into the mechanism of immune cell killing by combining live cell analysis and flow cytometry into a single workflow.

Full List of Incucyte Reagents & Consumables

Download our list of popular IncuCyte® reagents, cell lines and consumables.

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