Application Note: Benchmarking of Two Mycoplasma Real-time PCR Kits suitable for Release Testing According EP 2.6.7

Overview

This benchmark study compares the capabilities of two different qPCR kits for the detection of Mycoplasma, namely, the Sartorius Microsart^® ATMP Mycoplasma qPCR kit and the CellSafe MycoQSearchTM qPCR kit. The EP 2.6.7 required sensitivity of 10 CFU / mL of selective species is tested using three different Sartorius Microsart^® Validation Standards (Acholeplasma laidlawii, Mycoplasma orale, Mycoplasma pneumoniae) and one CellSafe Standard (Mycoplasma arginini).