QC BioAssays and Serial Dilution Guide | Sartorius

Application Guide: Preparation of Bioassays & Serial Dilutions in Microplates

Last updated: June 2024

Overview

Bioassay techniques are pivotal in quantifying and characterizing the effects of substances on biological systems. Utilized extensively in biotherapeutics, diagnostics, and environmental sciences, these assays range from ELISA for protein and antibody detection to flow cytometry for cell health analysis. Bio-layer interferometry and cell-based assays further elucidate drug-receptor interactions. Precision in sample preparation, including meticulous pipetting and the use of leachable-free consumables, is imperative to maintain assay integrity. Rigorous biocontamination prevention and the employment of pure lab water are also essential to ensure accurate, reproducible results, thereby facilitating advancements in drug development and biomarker discovery.


Download the documentation in this guide to support safe cell sample preparation and minimal deviations in QC bioanalytics.


  • Document type: Application Guide
  • Page count: 2
  • Read time: 5 minutes


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Key Takeaways

  • Reformat Samples from 96 to 384 well plates
  • Improve serial dilution accuracy
  • Prevent Mycoplasma contamination
  • Preserve protein recovery
  • Convert ELISA assays

This Resource is Designed for:

  • Quality Control
  • Method Development
  • Analytical Excellence
  • Quality Assurance
  • Bioanalytical Lab

Applications Supported:

  • Sample Preparation
  • Serial Dilution in Microplate
  • Microplates
  • Mycoplasma Removal
  • Cell-based Bioassays 2D, 3D
  • ELISA
  • AAV Titer Quantification

Complete the Form to Access the Application Guide