Quantitation of Fc-Fusion Protein in Cell Culture Supernatants | Sartorius

Application Note: Quantitation of Fc-Fusion Protein in Cell Culture Supernatants

Overview

The cell line development group at Biogen IDEC needed a robust assay for the measurement of Fc-fusion protein (Protein 1) in crude cell culture supernatants. The group had historically used HPLC for protein quantitation during screening and selection of promising mammalian clones at every scale-up step, from 96- well microplates to 3L-bioreactors. They wanted to replace the HPLC method due to its low throughput, cumbersome sample processing, and long run times with a higher-throughput alternative that provided accurate, reliable results.

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