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Handbook: 6th Edition Live-Cell Imaging and Analysis
Author: Lindy O’Clair, Jenna Shean, Bernadett Kolozsvari, et al. | Last updated: Oct 03, 2022
The Live-Cell Imaging and Analysis Handbook is a comprehensive reference guide for live-cell analysis technologies, focusing on the complete suite of Incucyte® live-cell instruments, assays, reagents, and integrated software analysis tools. This publication is updated annually to reflect the latest Incucyte® offerings.
The 6th edition handbook focuses on artificial intelligence (AI) and new AI-driven software available for the Incucyte® Live-Cell Analysis Systems. The new Incucyte® AI Confluence Analysis (now offered with the Incucyte® Base Analysis Software), is our first to use neural network that was trained using input from a wide range of cell types. It can accurately segment cells across different cell types and morphologies, greatly simplifying image processing to a single analysis. This handbook also features the new the new Incucyte® AI Cell Health Analysis Software Module, which uses the same approach for label-free, AI-driven segmentation and quantification of live versus dead cells.
This new edition also introduces genetically encoded kinetic biosensor assays for analyzing cancer cells, including the Incucyte® Kinase Akt Assay for measuring dynamic changes in Akt activity, a serine/threonine kinase that is upregulated in various cancers. Other applications covered include cell culture quality control assays and kinetic assays for cell health and proliferation, cell function, cell movement and morphology, and assays for complex 3D models such as organoids.
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