Unisart^® Nitrocellulose Membranes for Lateral Flow Assays

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Our Unisart^® nitrocellulose membranes are specifically designed to be the ideal solid phase for all diagnostic lateral flow immunoassays. Every step in the Unisart^® membrane manufacturing process has been optimized in order to obtain a reproducible 3D membrane structure with a clean and defect-free membrane surface. The homogenous structure of our membranes ensures a controlled and uniform lateral wicking of samples and reagents across the membrane which leads to improved accuracy and reproducibility of lateral flow test results.

Application Note

Surfactants Selection Guide for Lateral Flow Assays

PDF | 2.0 MB

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Brochure

UniSart^® Nitrocellulose Membranes - The Substrate of Choice for Protein Assays

PDF | 9.1 MB

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White Paper

Consistency in Physical Data of Unisart^®CN Membranes

Download the application note to learn more about the science behind the high quality of Sartorius Unisart^®️ CN Membranes.

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Consistency matters

Reliable capillary speed, high protein binding, homogenous thickness and a smooth and defect-free membrane surface are essential to obtain high quality lateral flow test strips. The exceptionally high inter- and intra-lot consistency of our Unisart^® membranes will help you to minimize the variability within lateral flow immunoassays and ensures accurate and precise results. Producing more consistent readouts improves test strip performance and reduces manufacturing costs.

Reliable membrane supply

In-depth know-how in nitrocellulose membrane manufacturing as well as relevant application expertise are driving us to continuously improve our state-of-the-art manufacturing equipment. As a technology leader, we guarantee reliable supply of high quality nitrocellulose membranes and also ensure availability of our Unisart^® membranes on large scale.

Quality made in Germany

We focus on quality in every step of our membrane manufacturing which is located in Göttingen, Germany. Our Unisart^® membranes are developed, produced and distributed according to a Quality Management System that is certified in compliance with DIN/ISO 9001. All products must pass our precisely defined in-house tests and meet our stringent quality control standards throughout all manufacturing steps.

Complete tracebility

All Unisart^® membrane products have a comprehensive and easy to read out labeling which allows convenient retrieval of all data compiled on materials used, production steps and QC tests. Quality assurance certificates guarantee full visual inspection and conformity to specifications for all Unisart^® lateral flow membranes.

Unisart^® Nitrocellulose Membrane Product Family

Membranes that make a difference

The nitrocellulose membrane is one of the most important components of a lateral flow rapid test and should be selected carefully. Especially the capillary speed of the membrane is very critical for lateral flow strip functionality as it affects sensitivity, specificity, response time and eventually costs of the assay. Each membrane type of our Unisart^® nitrocellulose membrane family has a specific range of capillary speed. Our Unisart^® CN 95 membrane is perfectly suited for high viscous samples and produces test strips with very short response times. Consequently Unisart^® CN 95 is the membrane of choice when a quick response is desirable. In contrast, test strips with our Unisart^® CN 180 DX membrane have the longest response time, but the assays will show the highest sensitivity. According to the application, reagents and manufacturing conditions our Unisart^® CN 110, CN 140 and CN 150 membranes provide a different optimum for speed, specificity and sensitivity. Our wide selection of Unisart^® membranes allows you to find the one that best meets the requirements of your diagnostic assay.

Solutions for Lateral Flow Assays & In Vitro Diagnostics Kit (IVD) Manufacturers

Sartorius is globally recognized for an expertise in biotherapeutics, including solutions for in vitro diagnostics kit (IVD) manufacturers. We can help you:
Design a standardized sample preparation protocol before diagnosis with tailored lab instruments and consumables

Get optimal performance characteristics on your lateral flow rapid tests with consistent and homogeneous UniSart^® membranes
Monitor and trace your critical Quality Control parameters
Accelerate your analyte discovery steps, development and production

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Membrane Specifications

Unisart^®	CN 95 backed	CN 110 backed	CN 140 unbacked	CN 140 backed	CN 150 backed	CN 180 DX backed
Membrane Material	cellulose nitrate	cellulose nitrate	cellulose nitrate	cellulose nitrate	cellulose nitrate	cellulose nitrate
Backing	100 µm polyester, clear	100 µm polyester, clear	none	100 µm polyester, clear	100 µm polyester, clear; 100 µm polyester, white	100 µm polyester, clear
Thickness (µm)	240 - 270	185 - 215	120 - 160	225 - 255	240 - 280	225 - 255
Capillary speed down web, purified water (s/40mm)	65 - 115	90 - 130	90 - 150	95 - 155	90 - 180	135 - 175
Visual appearance	All Unisart^® membranes offer a white, flat and smooth surface, free of macroscopic defects and foreign matter. They are all 100% inspected by backlight inspection.
Wettability	Precise amount of anionic surfactant allows for fast wetting

Roll Specification

Plastic roll core	76.8 mm, 3“
Certificate of analysis	provided with each delivery
Membrane width	17—300 mm (+/- 0.5mm)

Membrane wound with yellow interleaving protection paper between the membrane layers.

Applications

Unisart^®	CN 95 backed	CN 110 backed	CN 140 unbacked	CN 140 backed	CN 150 backed	CN 180 DX backed
Backing	100 µm clear	100 µm clear	none	100 µm clear	100 µm clear, 100 µm white	100 µm clear
Belt side as application side	no	no	yes	no	yes	no
Background clearing	very fast	very fast	fast	fast	fast	fast
Time–to-signal	fast	fast	medium	medium	medium	medium
Quick response tests	best suitable	suitable, depending on migration distance	suitable, depending on migration distance	suitable, depending on migration distance	suitable, depending on migration distance	suitable, depending on migration distance
Sensitivity	good	good	very good	very good	very good, can be enhanced by using white backing	excellent
Viscous samples	very good	good	good	good	good	good
Particle loaded samples	very good	very good	good	good	good	good
Backing as barrier against card glue	yes	yes	no, cards need to be selected carefully	yes	yes	yes
Contact dispensing equipment	suitable	suitable	suitable	suitable	suitable	suitable
Non- contact dispensing equipment	suitable	suitable	suitable	suitable	suitable	suitable
Suitable for reel-to-reel manufacturing	suitable	suitable	needs careful handling	suitable	suitable	suitable

Ordering Information

Unisart^® Membrane	Backing Polyester	Width (mm)	Length (m)	Max. Splices	Units/ Box	Catalog Number
CN 95 backed
CN 95	100 µm clear	20	50	0	5 rolls	1UN95ER050020WSB
CN 95	100 µm clear	25	50	0	5 rolls	1UN95ER050025WSB
CN 95	100 µm clear	30	50	3	5 rolls	1UN95ER050030—B
CN 95	100 µm clear	20	100	3	1 roll	1UN95ER100020NT
CN 95	100 µm clear	25	100	1	5 rolls	1UN95ER100025KSB
CN 95	100 µm clear	25	100	3	5 rolls	1UN95ER100025NTB
CN 95	100 µm clear	28	100	3	5 rolls	1UN95ER100028NTB
CN 95	100 µm clear	30	100	3	5 rolls	1UN95ER100030—B
CN 95	100 µm clear	35	100	3	5 rolls	1UN95ER100035NTB
CN 95	100 µm clear	40	100	0	1 roll	1UN95ER100040WS
CN 95	100 µm clear	70	100	0	1 roll	1UN95ER100070WS
CN 110 backed
CN 110	100 µm clear	20	100	3	5 rolls	1UN11ER100020NTB
CN 110	100 µm clear	25	100	3	5 rolls	1UN11ER100025NTB
CN 110	100 µm clear	25	100	3	1 roll	1UN11ER100025NT
CN 140 unbacked
CN 140	none	200	0.25	0	2 sheets	1UN14AS25020001
CN 140	none	20	50	3	5 rolls	1UN14AR050020—B
CN 140	none	25	50	3	5 rolls	1UN14AR050025—B
CN 140	none	20	100	3	5 rolls	1UN14AR100020NTB
CN 140	none	20	100	0	5 rolls	1UN14AR100020WSB
CN 140	none	25	100	3	5 rolls	1UN14AR100025NTB
CN 140	none	25	100	0	5 rolls	1UN14AR100025WSB
CN 140	none	35	100	3	5 rolls	1UN14AR100035NTB
CN 140	none	40	100	3	1 roll	1UN14AR100040NT
CN 140	none	270	100	3	1 roll	1UN14AR100270NT
CN 140 backed
CN 140	100 µm clear	20	50	3	5 rolls	1UN14ER050020—B
CN 140	100 µm clear	25	50	3	5 rolls	1UN14ER050025—B
CN 140	100 µm clear	25	50	0	5 rolls	1UN14ER050025WSB
CN 140	100 µm clear	17	100	3	1 roll	1UN14ER100017NT
CN 140	100 µm clear	18	100	3	5 rolls	1UN14ER100018NTB
CN 140	100 µm clear	20	100	3	1 roll	1UN14ER100020NT
CN 140	100 µm clear	20	100	3	5 rolls	1UN14ER100020NTB
CN 140	100 µm clear	25	100	3	1 roll	1UN14ER100025NT
CN 140	100 µm clear	25	100	3	5 rolls	1UN14ER100025NTB
CN 140	100 µm clear	28	100	3	5 rolls	1UN14ER100028NTB
CN 140	100 µm clear	30	100	3	5 rolls	1UN14ER100030—B
CN 140	100 µm clear	35	100	3	5 rolls	1UN14ER100035NTB
CN 140	100 µm clear	40	100	0	1 roll	1UN14ER100040WSB
CN 140	100 µm clear	70	100	0	1 roll	1UN14ER100070
CN 150 backed
CN 150	100 µm clear	20	50	3	5 rolls	1UN15LR050020NTB
CN 150	100 µm clear	25	50	3	5 rolls	1UN15LR050025NTB
CN 150	100 µm clear	20	100	3	5 rolls	1UN15LR100020NTB
CN 150	100 µm clear	25	100	3	1 roll	1UN15LR100025NT
CN 150	100 µm clear	25	100	3	5 rolls	1UN15LR100025NTB
CN 150	100 µm white	25	100	3	5 rolls	1UN15WR100025NTB
CN 180 DX backed
CN 180 DX	100 µm clear	20	100	3	5 rolls	1DX18ER100020NTB
CN 180 DX	100 µm clear	25	100	3	5 rolls	1DX18ER100025NTB

Customized dimensions of Unisart^® nitrocellulose membranes are also available upon request.

UniSart^® Nitrocellulose Membrane FAQs

What is the shelf-life of the membrane?

If stored according to our storage recommendations, UniSart^® Membranes have a shelf-life of 3 years after their manufacturing date. UniSart^® Microarray Slides have a shelf-life of 1 year after their manufacturing date. The shelf-life is indicated on each Certificate of Analysis.

What storage conditions are recommended?

It is recommended to store UniSart^® membranes at a constant temperature between 15 – 25°C and a relative humidity between 40 – 60 %. In any case, the membrane should be kept away from open fire, heat sources, light and chemical vapors of any kind.

What packaging is recommended for the storage of membrane rolls?

It is recommended to keep the membrane rolls in their original packaging and keep it closed in a dedicated storage area. The membrane roll with paper separator is packed in a primary packaging (plastic pouch). The article in primary packaging is packed in a secondary packaging (cardboard box).

What is the yellow paper in between the membrane layers?

The paper separator has an important safety function. Therefore, it must be ensured that the paper separator is not removed from the membrane rolls before processing. The paper separator significantly reduces the decomposition rate of our UniSart^® membranes. All UniSart^® membranes are exclusively sold containing this paper separator.

What must be considered for handling of the membrane?

When handling UniSart^® membranes ensure sufficient ventilation and avoid direct skin contact to prevent contamination of the membrane. Furthermore, prolonged direct contact to any material that can release chemicals should be avoided. These substances might alter important membrane properties such as wettability.

Do not store more membrane than needed for your daily production in your working area.

How can I order a membrane roll?

Sartorius offers five different membrane types within the UniSart^® CN Membranes portfolio with a broad range of different formats. Download the brochure.

Some of the products are available on the Sartorius eShop and can be ordered online. If your desired item is not included, please contact our team via unisart@sartorius.com.

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Unisart® Nitrocellulose Membranes for Lateral Flow Assays