iQue Qbeads^®

Enable screening and profiling studies with rapid quantification of secreted proteins in 96 or 384-well plates

Figure 1. Quantify cytokine secretion in minimal time using a 384-well assay format.

PBMCs (80K/well) from multiple donors were seeded alone (single donor controls) or in combination (8 donor pairs in total) in a Mixed Lymphocyte Reaction (MLR) assay. Cells were treated with several concentrations of an anti-CTLA4 checkpoint inhibitor antibody. Heatmap representation of secreted IFNγ across the 384-well assay plate. High speed analysis of a 384-well plate completed in 20 minutes.   

Analysis of up to 30 secreted protein concentrations in a single sample using customized or pre-configured panels.

Figure 2. Analyze data quickly and easily using the iQue Forecyt^® software system.
  
Dendritic cells were seeded with encoded CD4+ T cells (3:1 T cell-to-DC  ratio) and treated with Pembrolizumab before being analysed on Days 2 and 6 using the iQue Qbeads^® Human Inflammation Panel Kit. Pre-defined analysis templates are provided with each kit for the included analytes. (A) Gating template used to separate Qbeads based on their unique fluorescent signature. (B) Baseline secretion of multiple inflammatory cytokines measured using Qbeads in a single well performed in duplicate. (C) Efficiently present clear data trends using iQue Forecyt^® heatmaps and concentration response curves of secreted proteins and cytokines. 

Multiplex iQue Qbeads^® with other iQue^® kits and/or antibodies for combined analysis of cell functions and subsets.

Figure 3. Multiplexed quantification of secreted signaling molecules and cellular functions.
  
T-cells were activated using either Dynabeads or CD3-CD19 BiTE (Bi-specific T-cell Engager) and the production of cytokines was captured and analysed using iQue Qbeads^® Plexscreen in addition to cellular viability (using the iQue^® Cell Membrane Integrity Kit) within the same well. Lower levels of cytokine production in the BiTE treated t-cells compared to Dynabeads correlated with increased target cell killing. (A) Heatmap showing secreted concentrations of TNFα with increasing concentration of t-cell activators. Concentration response curves of IFNγ secretion after activation of t-cells with Dynabeads (B) and BiTE (C). Cellular viability with Dynabeads (D) and BiTE (E).    

Reduce reagent costs and time to answer – use as little as 2 µL conserving precious sample with no wash protocol.

Figure 4. iQue Qbeads^® Plexscreen kit workflow.

(A) Schematic showing the simple workflow for measuring analyte concentrations using iQue Qbeads^® Plexscreen. (B) The iQue Qbeads^® assay builder provides a flexible assay format which facilitates analysis of up to 30 secreted protein concentrations in a single well. (C) Distinct identification of iQue Qbeads^® and cells characterized by iQue Forecyt^® software population gating.