Advances in Induced Pluripotent Stem Cell Research
Stem cell research, takes advantage of the power and flexibility of pluripotency and self-renewal.Induced pluripotent stem cells (iPSCs) are capable of differentiating into somatic cells, and are infinitely expandable in culture. These characteristics make iPSCs valuable as a tool for developing cell culture models for the study of diseases and drug development, and also for the development of personalized therapeutics.
Growth factors and cytokines in iPSC culture media will inevitably degrade over time, at differing rates, so optimization of these key components is a requisite for maintenance of homogeneity during growth and expansion. Hence knowledge of the precise concentrations, and delivery of the various growth factors and cytokines is vital.
This eBook provides a collection of data and methods for culturing, optimization, and characterization of iPSCs using cytometry as an analysis tool.
Page count: 54Read time: 60 Minutes
A range of methods and techniques, from the optimization of iPSC culture and differentiation workflows, to advanced methods for the characterization of surface and intracellular marker expression is highlighted, including:
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