Combining Immuno-Affinity Chromatography and Filtration to Improve Specificity and Size Distribution of Exosome-Containing EV Populations Application Note

App Note: Combining Immuno-Affinity Chromatography and Filtration

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Application Note: Combining Immuno-Affinity Chromatography and Filtration to Improve Specificity, Size Distribution of Exosome-Containing EV Populations 

Overview

Enrichment of small extracellular vesicles of endosomal origin (exosomes) with average diameters of less than 150 nm is a prerequisite for investigation of their exact biological functions. In this application note, an experimental setup is presented, which combines Fab TACS® immuno-affinity chromatography based on the Strep-tag® technology (IBA Lifesciences) with subsequent filtration using Minisart® High Flow syringe filters (Sartorius).

  • Page Count: 7
  • Read Time: 15 minutes
  • Last Updated: March 2023

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This Application Note Demonstrates:

  • The average hydrodynamic diameter of CD9+ or CD81+ EVs enriched by affinity chromatography from HEK293 cell culture supernatant or plasma can be effectively reduced by eluate filtration; thereby, EV populations with a small size distribution (30 – 150 nm) are created
  • Impurities caused by microvesicles, apoptotic bodies and lipoproteins other enrichment techniques (e.g., differential and density gradient ultracentrifugation, ultrafiltration and size exclusion chromatography) struggle with are avoided with our experimental setup

This Resource is Designed for:

  • Life Science Research
  • Cell Culture Labs

Applications Supported:

  • Immuno-affinity chromatography
  • Syringe filtration
  • Nanoparticle tracking analysis (NTA)

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