Lab Filtration: TFF For Rapid mAb Concentration- Protocol | Sartorius

Lab Filtration: TFF For Rapid mAb Concentration

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Concentrating mAbs by Tangential Flow Filtration with Vivaflow® Cassettes Protocol

Last updated: February 2023

Overview

This lab filtration protocol describes the use of Vivaflow® TFF cassettes for concentrating antibodies - including mAbs and IgGs,- from clarified cell culture supernatants. This economical, plug-and-play solution makes tangential flow filtration more accessible to research labs for fast and easy ultrafiltration | diafiltration of larger sample volumes.


  • Document type: Protocol
  • Page count: 3
  • Read time: 4 minutes


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Key Takeaways

  • A list of materials needed for antibody sample clarification, concentration and purification
  • General guidelines, including tips on speeding up the process even further
  • Visual overviews and step-by-step instructions of the complete workflow
  • Exemplary data showing rapid reduction in sample volume during the concentration step

This Resource is Designed for:

  • Researchers in laboratories expressing and purifying antibodies (IgGs, mAbs, etc.) and other proteins for structural and functional analyses, and biologics discovery/cell line/molecule development

Applications Supported:

  • Cell culture clarification
  • Antibody concentration
  • Antibody purification
  • Tangential flow filtration
  • Crossflow ultrafiltration
  • Membrane chromatography
  • Protein A affinity chromatography

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