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Effectively Removing Mycoplasma Strains Using Vacuum Filtration
Read time: 12 min | Authors: Helena Windsor, Florian Hebenstreit, Klaus Schöne
Preventing mycoplasma contamination is critical when working with cell cultures in order to ensure accurate, reproducible results in experimental trials and production of biological targets. To minimize risk of contamination, it is recommended to implement a number of measures, such as aseptic working, use of mycoplasma-free tested reagents/media and regular mycoplasma testing. In addition, filtration of media prepared from dry media is an essential prevention strategy.
In this application note, it was shown that tested Sartolab® RF vacuum bottle-top filters with a pore size of 0.1 μm can effectively remove the mycoplasma strains A. laidlawii and M. hyorhinis. Cell culture media DMEM, F17, and RPMI + 10% FBS spiked with the respective mycoplasma strains were used to simulate typical conditions during filtration.
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Figure 1. Experimental Setup for Filtration of Challenge Suspension
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