Urine Protein Concentration with Vivaspin^®

Overview

Measurement of proteins in urine is important for the diagnosis and monitoring of a variety of diseases and disorders.

Normally proteins larger than 100 kDa, such as immunoglobulins, are retained in blood and much smaller molecules (<10 kDa) pass freely into the urine. Intermediate sized molecules, such as albumin (~69 kDa) and free light chains (FLC) (~25 kDa), will pass into urine to varying degrees and then usually be re-absorbed by the nephron tubular system. However, these proteins can also be excreted in urine as the result of several conditions. To enable accurate and early diagnosis, it is often necessary to concentrate the protein content of urine samples prior to analysis.

 Vivaspin^® centrifugal devices are ideal for this purpose, combining fast ultrafiltration with high concentration factors and recoveries, to reach the sensitivity required for accurate detection in subsequent electrophoretic techniques

• Document type: Application Note
• Page count: 6
• Read time: 5 minutes

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