Incucyte^® Spheroid Analysis Software Module

Incucyte^® Spheroid Analysis Software Module (Cat. No. 9600-0019)

Analyze growth, viability and invasion of single spheroids in round-bottom, multi-well format or measure multi-spheroids in flat bottom plates to detect changes in growth and viability inside your tissue culture incubator.

Incucyte^®Spheroid Analysis Software Overview

The Incucyte^® Spheroid Analysis Software Module enables kinetic acquisition and objective assessment of spheroids within a physiologically relevant environment.

The integration of optimized protocols and Incucyte^® software enables you to standardize your entire spheroid workflow, from generation, manipulation, and analysis, using imaged-based, label-free or fluorescence measurements. Characterize and assess spheroid growth or invasion and morphology during cultures and probe the effects of treatments through unbiased assessment of size, count and morphology. Incucyte^® purpose built software automatically monitors and quantifies changes in two different assays models, depending on your scientific question:

Scaffold-free grown in round-bottom ULA plates

Single spheroids tumor models to evaluate growth, shrinkage and death in 96- and 384-well formats
Single spheroid invasion tumor model to quantitate invasive growth and shrinkage in a 96-well format*

Learn more about Incucyte^® Tumor Spheroid Assays and Spheroid Invasion

Scaffold-based grown in flat bottom plates

Multi- spheroids (MS) grown on a layer of Matrigel^® or embedded in Matrigel^® on a base (label-free) to quantify tumor spheroid formation, growth and health in a 96-well format*. Fluorescence image acquisition only available using the MS on a Layer of Matrigel^® model.

Learn more about Incucyte^® Multi-Spheroid Assays

_{*Not available for the Incucyte}^®_{Zoom- or FLR Live-Cell Analysis Systems}

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Incucyte^® Spheroid Analysis Software Features

Kinetically monitor and quantify spheroid changes overtime with purpose-built acquisition and analysis software

Figure 1: Process images of every well with easy to use guided interface (A) and assess experimental conditions using Incucyte^® vessel view (B). Integrated kinetic analysis shows powerful insight for the characterization of advanced cell models (C).

Generate Reproducible, Quantitative Data

Lab-tested protocols for Single- or Multi-Spheroid Assays, high quality images, and unbiased analysis deliver robust data suitable for pharmacological analysis.

MCF-7

MS on a Layer of Matrigel^® (10x magnification)

MDA-MB-231

MS on a Layer of Matrigel^® (10x magnification)

MS Embedded in Matrigel^® on a Base (4x magnification)

Figure 2: Representative videos indicating label-free multi-spheroid growth and reveal differential spheroid morphologies over time. MCF-7 and MDA-MB-231 cells were seeded in media on a top of Matrigel^® layer or embedded in Matrigel^® on a top of base layer (1K cells/well) and allowed to form multi-spheroids (3 days). Time-lapse videos monitoring spheroid growth over time (0 – 7 days post formation) demonstrate morphological differences between round (MCF-7) and stellate (MDA-MB-231) multi-spheroids.

Learn more about Protocols and Assays: Incucyte^® Tumor Spheroid Assays or Incucyte^® Multi-Spheroid Assays

Unlock Your Productivity

Automatically acquire, analyze, and graph thousands of images from up to six 96- or 384-well plates in parallel and get to answer faster.

Figure 3: Perform robust, reproducible pharmacological analysis in physiologically relevant conditions. MCF-7-Nuclight red multi-spheroids were formed over 3 days prior to 7-day treatment with known cytotoxic compounds. Time-course plate views enable rapid visualization of treatment effects on both spheroid size (Total BF Area) and viability (red FLU Intensity within BF Boundary). Concentration response curves represent area under curve (AUC) analysis of the time-course data 0 - 7 days post-treatment. All compounds caused a concentration dependent inhibition of growth and viability with rank order of potency CMP > CHX > OXA.

Ordering Information

Product	Qty.	Cat. No.
Incucyte^®Spheroid Analysis Software Module	1 module	9600-0019
Incucyte^®Cytolight Green Lentivirus (puro)	0.6 mL	4481
Incucyte^® Cytolight Red Lentivirus (puro)	0.6 mL	4482
Incucyte^® Nuclight Green Lentivirus (bleo)	0.2 mL	4626
Incucyte^® Nuclight Green Lentivirus (bleo)	0.6 mL	4477
Incucyte^® Nuclight Red Lentivirus (bleo)	0.2 mL	4627
Incucyte^® Nuclight Red Lentivirus (bleo)	0.6 mL	4478
Incucyte^® Nuclight Green Lentivirus (puro)	0.2 mL	4624
Incucyte^® Nuclight Green Lentivirus (puro)	0.6 mL	4475
Incucyte^® Nuclight Red Lentivirus (puro)	0.2 mL	4625
Incucyte^® Nuclight Red Lentivirus (puro)	0.6 mL	4476
Incucyte^® Nuclight Orange Lentivirus (puro)	0.2 mL	4771
Incucyte^® Nuclight NIR Lentivirus (puro)	0.2 mL	4805
Incucyte^® Annexin V Green Dye	1 vial	4642
Incucyte^® Annexin V Red Dye	1 vial	4641
Incucyte^® Annexin V Orange Dye	1 vial	4759
Incucyte^® Annexin V NIR Dye	1 vial	4768
Incucyte^® Cytolight Rapid Green Dye	1 vial	4705
Incucyte^® Cytolight Rapid Red Dye	1 vial	4706