What Is Immune Cell Clustering and Proliferation?

• Monitor proliferation and cell-cell clustering interactions in real time, without removing your cultures from the incubator
• Automated imaging and analysis – count label-free clusters using intuitive IncuCyte^® image analysis software
• Gain phenotypic insight from images and movies

Figure 1. Label-free detection of T cell aggregation. Activation of PBMCs with anti-CD3 antibody and IL-2 induces T cell aggregation or "clustering". User-friendly IncuCyte^® software enables accurate quantification of cell clustering over time (blue mask).

• Reveal how treatments affect the kinetics of immune cell clustering and proliferation
• Cultures remain undisturbed and in your incubator throughout the experiment

Figure 2. Measure clustering in real time. Automated time-course analysis reveals treatment effects. Increasing the concentration of anti-CD3 antibody from 0.01 to 10 µg/ml in the presence of IL-2 (10 ng/ml) promotes T cell clustering

• Measure proliferation and clustering without the need for labels
• Set up and walk away – fully automated image capture and analysis
• Compatible with complementary flow cytometry and end-point biochemical measurements – simply harvest cells/supernatants when the assay is complete

Real-time, label-free monitoring of PBMC proliferation.
The Incucyte^® Live-Cell Analysis System automatically quantifies proliferation of PBMCs (yellow mask) activated with anti-CD3 antibody and IL-2 over a period of 144 hours. The full time course of PBMC proliferation is shown in the presence of anti-CD3 antibody and IL-2, anti-CD3 antibody alone, and in the absence of activators.