Octet® BLI for Antibody Characterization & Quality Control in IVD Development
Authors: Shuo Liang and David Apiyo | Last updated: November 2023
A lateral flow assay (LFA) is one of the most common immunoassay formats for in vitro diagnostics (IVD), and antibodies play a critical role. It is important to select the best performing antibodies and to implement analytical techniques that can ensure consistent performance of these antibodies from batch to batch, as the quality attributes of these antibodies and their interaction with antigens affect the test performance of LFAs.
Download the poster to explore the role of Octet®️ BLI systems in LFA development and quality control. Utilizing Bio-Layer Interferometry (BLI) technology, these instruments provide a real-time, label-free approach for optimal antibody characterization in comparison to end-point ELISA. With scalable high-throughput antibody-antigen interaction analysis, optimal antibody selection and quality control are fast and reliable. Hence, Octet®️ BLI systems are essential for LFA development for IVD. Combined with Sartorius' advanced membrane technologies, we offer a comprehensive solution to accelerate the development and manufacturing of LFAs.
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Critical steps in the development and production of an LFA
Octet® BLI systems workflow for antibody characterization
Protein-protein binding kinetics analysis on Octet® BLI systems
Antibody-antigen binding specificity assessment on Octet® BLI systems
Using Octet® BLI systems in batch-to-batch quality check and lot release
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