Vivaflow and Vivaspin Workflow in Protein Research Laboratories
Application Note: Vivaflow® and Vivaspin® Workflow in Protein Research Laboratories

Fast Protein Purification

By Ultrafiltration and Membrane Chromatography

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Application Note: Vivaflow® and Vivaspin® Workflow in Protein Research Laboratories

This application note presents a fast, easy workflow protein purification from clarified cell culture media. Vivaflow® TFF cassettes were used to concentrate and re-buffer a mock cell culture sample prior to protein purification on Vivapure® cation exchange membrane adsorbers. A final ultrafiltration and diafiltration step with Vivaspin® centrifugal ultrafilters was used to concentrate and desalt the purified protein.

In our example, the total purification process time was less than four hours. Furthermore, with re-usable Vivaflow® TFF cassettes and no need for specialist FPLC equipment to perform ion exchange chromatography, this is a rapid and cost-effective purification strategy for virtually any macromolecule in research and development laboratories.

Overview

Document type: Application Note
Page count: 6
Read time: 5 minutes
Audience: Researchers in laboratories expressing and purifying proteins for structural and functional analyses, and biologics discovery/cell line/molecule development
Applications covered: Protein purification, Protein concentration, Protein diafiltration, Buffer exchange, Tangential flow filtration, Crossflow ultrafiltration, Ion exchange purification, Membrane chromatography
 

Included in this Application Note:

  1. Example workflow for the purification of lysozyme from 2 L of cell culture media
  2. Detailed results, including process times and target molecule recoveries
  3. Suggested products for your purification process

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