High Precision Streptavidin 2.0, SAX2, biosensors have been designed to minimize variability in biotinylated ligand immobilization and to enable the development of ligand binding kinetics or protein quantitation assays where confidence in precision is critical. SAX2 biosensors are qualified to limit variability in ligand immobilization to less than 4% within the same lot and less than 20% between different lots, which translates into better reproducibility for antigen or analyte binding in a kinetics characterization application.

In this poster, we show an example workflow for the use of SAX2 biosensors in the immobilization of a biotinylated ligand and the subsequent use for a potency assay as established at Emergent BioSolutions, Inc., and a robust kinetics assay suitable for downstream applications. At Emergent BioSolutions, a biotinylated antibody and antigen sample pair were used with three different manufacturing lots of SAX2 biosensors. The assay was run on an Octet RED96e system. We also show other examples where SAX2 biosensors were used to establish low variability in a kinetics characterization assay and a custom quantitation assay.