Assess neuronal activity like never before with the IncuCyte® S3 Neuronal Activity Assay. The generation and maturation of neuronal networks are necessary characteristics of neurogenesis, providing confirmation that the cellular processes required for proper neuronal function have been developed. Recent advances in human induced pluripotent stem cell (hiPSC)-derived neurons offer promise as a powerful in vitro model strategy for the study of both the basic mechanisms of development as well as complex disorders of the human nervous system. However, characterization and optimization of these models as they differentiate, mature, and become functionally active are limited by current techniques. The IncuCyte® Neuronal Activity Assay is an end-to-end solution consisting of instrumentation, software, and reagent that enables unprecedented access to complex, neuronal activity measurements enabling functional insight into neuronal cell models.