Application Note: Kinetic Quantification of Cell Proliferation Using Live-Cell Analysis

Overview

Cell proliferation assays are fundamental to various research fields, including cancer therapeutics, developmental biology, and drug safety. These assays are instrumental in understanding the complex signaling pathways that drive tumor progression, as evidenced by the extensive literature on the subject. Despite their widespread use, cell proliferation assays present technical challenges that must be addressed to obtain accurate and meaningful data.

Traditional methods of cell proliferation analysis often require endpoint measurements that can miss critical biological events. Moreover, the need to maintain non-perturbing conditions over extended periods is crucial for obtaining high-quality results. Temporal monitoring is essential to capture the dynamic nature of cell growth, and this necessitates methodologies that allow for continuous data collection without disrupting the cellular environment.

The Incucyte^® Live-Cell Analysis System offers a solution to these challenges by enabling real-time, non-invasive monitoring of cell proliferation. This system utilizes time-lapse imaging, integrated image analysis, and immediate data visualization to track the behavior of living cells over extended periods. The continuous data collection facilitated by live-cell analysis eliminates the need for multiple endpoint assays and reduces the risk of missing significant biological events.

• Document type: Application Note
• Page count: 5
• Read time: 15 minutes

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