Octet® BLI Resources Library | Sartorius

Octet® BLI Resource Library

Research the Industry Standard for Label-Free of Biomolecular Interactions Analysis (BIA)

Research the Industry Standard for Label-Free of Biomolecular Interactions Analysis (BIA) - The Octet® BLI platform enables label-free biomolecular interaction analysis based on Bio-Layer Interferometry (BLI) technology. Download the latest application notes, infographics, eBooks, and more to support your label-free research and development.

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Featured Octet® Resources

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Illustration - Drug Tolerance Assay ADA Present - Drug Excess Present using Octet BLI System
Application Note

Advances in Immunogenicity Evaluation - Paving the Way for Safer Biolo...

Anti-drug antibodies (ADA) can modify the drug’s pharmacodynamics (PD) and/or pharmacokinetics (PK), making it crucial to detect them through an immun...

A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...

End of End-Point Assays cover
White Paper

The End of End-Point Assays

Faster time-to results allows assessment of accurate and precise data earlier in the workflow

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Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment

Application Guides (17)

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harmonizing regulatory guidelines for assay validation application note cover
Application Guide

Harmonizing Regulatory Guidelines for Assay Validation

Current regulatory guidelines for assay validation were harmonized for kinetic-based assay to derive a common set of parameters.

Application Guide

Label-free to Decipher CRISPR/Cas Mechanisms

Octet® BLI to measure and characterize CRISPR/Cas mediated binding and Gene activation

A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...

Octet® BLI Workflows in Small-Molecule Interaction Discovery and Characterization
Application Guide

Small-Molecule Interaction Discovery and Characterization

With Octet® BLI Workflows

Cell Line Development: Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform
Application Guide

Cell Line Development

Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform

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Application Guide

Determining Nanoparticle Vaccine Antigenic Efficiency

Nanoparticle (NP) vaccine and antigen delivery platforms have emerged as a promising approach due to their ability to interact with immune components

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Application Guide

Stability, Formulations and Aggregation Studies

Expanding Octet® Applications in Downstream Biologics Characterization

Application Guide

Accurate Measurements on the Octet® Platform

Without Added Sample Purification Steps

Advancing Development of Coronavirus Vaccine and Therapeutics cover
Application Guide

Advancing Development of Coronavirus Vaccine and Therapeutics

BLI Technology Plays a Vital Role in the Race for a SARS-CoV-2 Treatment

Application Guide

Recent Insights into Covid-19 Binding Epitopes

Researchers are using the Octet® system to understand the virus's binding mechanisms as a first step in the development of a vaccine

Octet® Systems: Modernize Biopharmaceutical QC Testing to Increase Efficiency
Application Guide

Modernize Biopharmaceutical Quality Control Testing

Quality control of biological products to support clinical trials and post market assay activities require the evaluation of multiple critical product...

Application Note cover
Application Guide

Label-Free Technologies

For Accurate Determination of Kinetics and Affinity Rate Constants

Application Guide

Ligand Binding Assays That Meet Compliance with Octet® BLI Systems

Octet® GxP packages support the GMP compliant implementation, including 21 CFR Part 11 software, validation packages, and biosensor validation support...

Cover: Characterizing Membrane Protein Interactions by Bio-Layer Interferometry
Application Guide

Characterizing Membrane Protein Interactions

By Octet® Bio-Layer Interferometry (BLI) Platforms

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Application Guide

A Simple Method to Determine Relative Potency (EC50)

Using Octet® BLI Technology

Simplifying manufacturing quality control cover
Application Guide

Simplifying Biotherapeutic Manufacturing and Quality Control

With Label-Free Biosensor Technology

Octet® Systems in Bioprocessing: Easy-to-Use and Cost-Effective Tools for Multiple Applications
Application Guide

Octet® Systems in Bioprocessing: Easy-to-Use and Cost-Effective T...

PDF 282.5 KB

Application Notes (29)

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Illustration - Drug Tolerance Assay ADA Present - Drug Excess Present using Octet BLI System
Application Note

Advances in Immunogenicity Evaluation - Paving the Way for Safer Biolo...

Anti-drug antibodies (ADA) can modify the drug’s pharmacodynamics (PD) and/or pharmacokinetics (PK), making it crucial to detect them through an immun...

Optimizing Kinetics Assays to Avoid Avidity Effects
Application Note

Optimizing Kinetics Assays to Avoid Avidity Effects

A fundamental understanding of the differences between affinity and avidity is essential when designing and developing assays

Application Note

Rapid, Automated, At-Line Adenovirus Quantitation Using Octet® BL...

Learn how we report the development of a rapid, high-throughput capsid assay for AV quantification on the Octet® BLI platform that demonstrates excell...

Octet R4 SARS-CoV-2 application note
Application Note

Octet® R4 Bio-Layer Interferometry Platform SARS-CoV-2

Biophysical Characterization of the Binding of SARS-CoV-2 to the ACE2 Protein Using the Octet® R4 Bio-Layer Interferometry Platform

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Application Note

Quantitation of Fc-Fusion Protein in Cell Culture Supernatants

The cell line development group at Biogen IDEC needed a robust assay for the measurement of Fc-fusion protein in crude cell culture supernatants.

Octet® AAVX Biosensors for Rapid and Direct Quantitation of AAV Capsids
Application Note

Octet® AAVX Biosensors

For Rapid and Direct Quantitation of AAV Capsids

Application Note

Measure Pharmacokinetics of Monoclonal Antibodies

Scientists from Elanco® use Octet® BLI to rapidly design and establish a preclinical PK method for analyzing canine antibodies

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Application Note

Customized Quantitation of Recombinant Therapeutic Proteins

Using Octet® SAX Biosensors

Analysis of FcRn-Antibody Interactions on the Octet® Platform
Application Note

Analysis of FcRn-Antibody Interactions on the Octet® Platform

Assay design recommendations and best practices for producing the highest quality of kinetic data.

Application Note

High-Throughput Assays for Epitope Binning and Cross-Competition Analy...

Epitope binning through cross competition assays is a powerful tool in evaluating functional diversity in antibody clones.

Analysis of Fc-Gamma Receptor-IgG Interactions on the Octet® Platform
Application Note

Analysis of Fc Gamma Receptor IgG Interactions on Octet® Platform

Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.

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Application Note

Second Generation Anti-Human IgG Fc Capture Biosensors

For Affinity Characterization of Human IgG-Antigen Interactions and Quantitation of Human IgGs or Human Fc Region Containing Proteins 

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Application Note

Mannose Glycans Content Screening for Human-mAb Samples

A novel approach for relative mannose content screening and ranking of human monoclonal antibody (mAb) samples using the Octet® GlyM Kit

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Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment

Validated Quantitation and Activity Assay of Antibody Fragment Molecule (Fab) for Process Development and Quality Control
Application Note

Validated Quantitation and Activity Assay of FaB

For Process Development and Quality Control

Application Note

MAb Quantitation Protein A HPLC vs Protein A Bio-Layer Interferometry

Rapid, accurate and cost-effective quantitation of monoclonal antibodies (MAbs) is essential for bioprocessing.

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Application Note

Potency Assay Development Qualification and Validation Strategies

A method for evaluating the binding of an Fc gamma receptor III (FcγR) molecule to the widely characterized NIST mAb.

A Rapid Method to Quantitatively Screen Bispecific Antibodies Using Protein A and Octet® His1K Biosensors
Application Note

Screen Bispecific Antibody Using Protein A and His1K Biosensors

Rapid quantitative screening of bispecific antibodies using Protein A and HIS1K biosensors

Application Note

Accelerating Process Optimization in Cell Line Development

This application note describes the power of using the Octet® platform along with Ambr® 15 Cell Culture to identify optimum yield conditions.

A Fast and High Precision Influenza Vaccine Potency Assay cover
Application Note

A Fast and High Precision Influenza Vaccine Potency Assay

An important understanding of vaccine development process performance, and for correctly scaling each process step.

Rapid, Automated, At-Line AAV2 Virus Quantitation Advances Bioprocessing in Gene Therapy
Application Note

Rapid, Automated, At-Line AAV2 Virus Quantitation Advances Bioprocessi...

PDF 1.0 MB
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Application Note

Enhancing Efficiency and Economics in Process Development and Manufact...

PDF 372.5 KB
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Application Note

Performing Quantitation Assays Using Octet® N1 System

PDF 101.2 KB
Instant Determination of Protein Presence Using Octet® N1 System
Application Note

Instant Determination of Protein Presence Using Octet® N1 System

PDF 762.6 KB
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Application Note

Fast Quantitation of Proteins and Antibodies Using the Octet® N1 Syste...

PDF 350.2 KB
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Application Note

Reducing Variability in Small Molecule Screening and Kinetics Applicat...

PDF 1.4 MB
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Application Note

Generating Reliable Kinetic Data For Protein Ligand Interactions

PDF 130.8 KB
Optimizing Protein-Protein and Protein-Small Molecule Kinetics Assays
Application Note

Optimizing Protein-Protein and Protein-Small Molecule Kinetics Assays

PDF 950.7 KB
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Application Note

CaptureSelect Affinity Ligands For Antibody Detection And Characteriza...

PDF 1.3 MB
Octet RH16 with Lab Tech
Application Note

Converting ELISA Assay Into Octet® Quantitation Assay

PDF 207.5 KB
Application Note

Futureproof your Lab with the Octet®️ R Series of Systems — Flexibilit...

PDF 5.5 MB

Brochures (3)

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Octet R Series System brochure cover
Brochure

Octet® R Series Systems

Accelerate your research with an upgradeable, label-free protein analysis platform

Label-free Assays in a Drop (N1 Brochure)
Brochure

Label-free Assays in a Drop (N1 Brochure)

PDF 2.9 MB
Octet®️ Systems Platform Brochure
Brochure

Octet® Systems - Superior Performance, Unrivaled Flexibility

PDF 1.3 MB

Case Studies (1)

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Case Study

Bispecific Antibodies: From Research to Clinical Application Case Stud...

PDF 222.1 KB

eBooks (8)

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Octet®️ BLI 21 CFR Part 11 Compliance Checklist eBook
eBook

GxP Compliance Without the Complexity

A comprehensive tool set for compliance makes the Octet® BLI platform an ideal analytical instrument in regulated environments.

eBook

Label-Free Techniques for Biologics Assessment

When investigating biological mechanisms and developing new therapeutics, BLI and SPR offer unique advantages

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eBook

Rapid and Efficient Measurement of Critical Quality Attributes and Par...

eBook: How Are Label-Free Technologies Used in Early Drug Discovery?
eBook

How Are Label-Free Technologies Used in Early Drug Discovery?

Modern analytical techniques designed for real-time analysis and high-throughput capabilities significantly reduce the time to the discovery

Label-Free Detection technologies, key considerations and applications cover
eBook

Label-Free Detection

Technologies, Key Considerations, and Applications

eBook

Accelerating the Development and Manufacture of Therapeutics

Establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032 requirements

Octet ebook cover
eBook

Overcoming the Challenges of Infectious Diseases

Explore the latest methodologies and strategies in the development of future vaccines fighting infectious diseases with label-free approaches

eBook

Accelerating Vaccines and Virus Research

Live Cell Analysis, Advanced Flow Cytometry and Label-Free Binding Analysis

Editorials (2)

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Editorial

Addressing the Challenges of QC Labs in Vaccine Manufacturing

In cell culture-based vaccine manufacturing processes, microbial contamination control and impurity testing are crucial elements of quality control.

Editorial

Advancing the Development of COVID-19 Neutralizing Antibodies

Learn more about how virus-neutralizing antibodies have proven to be a key tool in the fight against SARS-CoV-2.

Flyers (6)

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Flyer

Octet® AT: For Octet® BLI Biosensor Transfer

PDF 736.8 KB
Flyer

AAV Capsid Titer Data 80% Faster Compared to ELISA

Bioprocess Five Additional Batches of AAV Samples Annually

Octet service plan
Flyer

Octet® Service Contracts - Protect Your Octet®, Pioneer and BLItz® Sys...

PDF 191.1 KB
Flyer

New Octet®️ R Series of Systems Flyer

PDF 523.8 KB
Octet top 10 reasons to choose BLI
Flyer

Top Ten Reasons to Choose Sartorius Octet®️ BLI Technology to Accelera...

PDF 167.1 KB
selection guide
Flyer

Octet® BLI Biosensor Selection Guide

PDF 404.8 KB

Infographics & Posters (6)

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Flyer

AAV Capsid Titer Data 80% Faster Compared to ELISA

Bioprocess Five Additional Batches of AAV Samples Annually

Infographic
Infographic

Understanding Covid-19 Vaccine and Therapeutic DevelopmenT

Learn more about how Octet® systems are used to advance Coronavirus research and vaccine development

Poster

Influenza Vaccine Titre Determination Bio-Layer Interferometry (BLI)

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly sca...

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Infographic

Octet® Bio-Layer Interferometry (BLI) Technology

Label-free alternative for characterizing protein-protein and drug-protein interactions in standard microtiter plate format

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Poster

High Productivity and Process Economy in GxP Applications

Quantitation assays must show precision, accuracy, linearity, and reproducibility

Poster

Octet® BLI Systems

Unmatched Versatility for Discovery, Development and Quality Control

Technical Notes (30)

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Technical Note

Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosen...

PDF 657.9 KB
Octet Kinetics Assay Method Development Guideline thumbnail
Technical Note

Octet® Kinetics Assay Method Development Guideline

Developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032

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Technical Note

Octet® AHC2 Biosensors

For Quantitation and Kinetic Characterization of Human Fc-Region Containing Proteins

Octet® High Precision Streptavidin 2.0 (SAX2) Biosensors
Technical Note

High Precision Streptavidin 2.0 (SAX2) Biosensors

Streptavidin is often deployed as the molecule of choice for covalent linking to solid surfaces for ligand binding assays applications.

Octet Kinetics assay technical note cover
Technical Note

Octet® BLI Quantitation Assays: Method Development Guideline

Design and develop biological assays that conform to USP <1032> for accuracy, precision, linearity and limit of quantitation (LOQ) to be establi...

CHO Host Cell Protein Detection
Technical Note

CHO Host Cell Protein Detection

Designed for a rapid high-throughput quantitation of residual CHO Host Cell Proteins (HCPs) in bioprocessing samples during production and development...

Octet ProL Biosensors technical note cover
Technical Note

Octet® ProL Biosensors

For Quantitation of Whole-Molecule Antibody and Antibody Fragments

Immunogenicity Assessment of Therapeutic Protein on the Octet®️ BLI Sy...

PDF 656.1 KB
Octet®️ BLI 21 CFR Part 11 Software Frequently Asked Questions (FAQ)

Octet®️ BLI 21 CFR Part 11 Software Frequently Asked Questions (FAQ)

PDF 1.2 MB
Technical Note

Analysis of FLAG Proteins on the Octet®️ BLI Platform Using SAX or SAX...

PDF 901.4 KB
Lab Products & Services

Human and Mouse IgG Subtype Identification Using the Octet®️ Platform

PDF 707.5 KB
Octet®️ AMC2 Biosensors: For Quantitation and Kinetic Characterization of Murine IgG Antibodies
Lab Products & Services

Octet®️ AMC2 Biosensors: For Quantitation and Kinetic Characterization...

PDF 948.9 KB
Octet GlyM Kit Technical Note
Technical Note

Octet® GlyM Kit

PDF 2.2 MB
Octet AAVX Biosensors for Quantitation of AAV Capsids
Technical Note

Octet® AAVX Biosensors for Quantitation of AAV Capsids

PDF 2.0 MB
Small Molecule Binding Kinetics
Technical Note

Small Molecule Binding Kinetics

PDF 2.7 MB
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Technical Note

Host Cell Protein Detection

PDF 952.5 KB
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Technical Note

Octet® Anti-Mouse IgG Fc Capture (AMC) Biosensors

PDF 678.2 KB
Amine Reactive Second-Generation (AR2G) Biosensors
Technical Note

Amine Reactive Second-Generation (AR2G) Biosensors

PDF 723.9 KB
Octet® Amine Reactive SecondGeneration (AR2G) Reagent Kit technical note
Technical Note

Octet® Amine Reactive Second-Generation (AR2G) Reagent Kit

PDF 369.1 KB
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Technical Note

Biotinylation of Proteins for Immobilization onto Streptavidin Biosens...

PDF 515.3 KB
Octet® GST Biosensor Kinetics Assays technical note
Technical Note

Octet® GST Biosensor Kinetics Assays

PDF 348.6 KB
Octet® GST Biosensor Quantitation Assays
Technical Note

Octet® GST Biosensor Quantitation Assays

PDF 600.2 KB
Octet® NTA Biosensor Kinetic Assays technical note
Technical Note

Octet® NTA Biosensor Kinetic Assays

PDF 589.8 KB
Octet® NTA Biosensor Quantitation Assays technical note
Technical Note

Octet® NTA Biosensor Quantitation Assays

PDF 551.2 KB
Anti-HIS (HIS2) Biosensor Quantitation Assays technical note
Technical Note

Anti-HIS (HIS2) Biosensor Quantitation Assays

PDF 630.9 KB
Technical Note

High Precision Streptavidin Biosensor (SAX) Quantitation and Kinetic A...

PDF 3.6 MB
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Technical Note

Anti-Penta HIS (HIS1K) Biosensors for Label-Free Analysis of HIS-Tagge...

PDF 1.5 MB
Rapid Analysis of Fab Fragments and IgG with Anti-Human Fab2G Biosensors
Technical Note

Rapid Analysis of Fab Fragments and IgG with Anti-Human Fab2G Biosenso...

PDF 1.8 MB
Residual Protein A Detection Kit
Technical Note

Residual Protein A Detection Kit

PDF 1.8 MB

Webinars (15)

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Webinar

A New Wave Biosensor Approach for AAV Process Development

REGENXBIO Inc. shares their experience in finding a high-throughput technique for AAV capsid concentration quantification.

Antibodies binding in high avidity structures around multivalent antigens illustration
Webinar

Affinity versus Avidity Webinar

The multivalent nature of antibodies can lead to avidity effects that skew binding affinity data. Hear from a protein analytics expert about avoiding...

Webinar

Antibody-Drug Conjugates (ADCs) Webinar

Simplifying the Characterization of the drug trastuzumab to Kadcyla®️ and Enhertu®️

Webinar

Reduce Cycle Times for AAV Quantification

See the Octet® AAVX Biosensor in comparison to an in-house assay on the Octet BLI platform.

drug target review webinar
Webinar

CHO for Accelerated Antibody Development

Explore applications of the CHO antibody simultaneous display and secretion technology

Webinar

Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Host Immunity

Cell surface Nucleocapsid (N) protein may play an important role in host adaptive immunity to SARS-CoV-2

Webinar

Rapid Characterization and Selection of SARS-CoV-2 Vaccine Candidates

Using Bio-Layer Interferometry Technology, we developed a set of assays to identify antibodies with SARS-CoV-2 reactivity

Webinar

Bio-Layer Interferometry as a Strategic Platform

To validate covalent proximity inducing small molecules with synthetic tumor immunotherapeutic applications

Webinar

Novel High-Throughput Glycoanalytics for Biopharmaceuticals

Improve the overall drug discovery process to increase the success rate of preclinical candidates.

Webinar

Integrating Octet® BLI into Early Antibody Discovery Workflows

Improve the overall drug discovery process to increase the success rate of preclinical candidates

Webinar

Characterizing Critical Reagents in Support of Drug Development

BLI technology for affinity characterization antibodies can benefit critical reagent workflows

Webinar

Fragment Based Drug Discovery of Co-Factor Inhibitors for p97

Virtual screening of molecular databases and biochemical/biophysical techniques

Webinar

Optimers™️ - Future Ready Antibody Mimetics

Discover the new versatile Octet®️ R series and the use of Optimer™️ binders to improve affinity purification of novel biologics

Octet R Series lab tech
Webinar

Drug Discovery with High-throughput Biomolecular Interaction Analysis

Learn how Octet® BLI works in therapeutic discovery and development, including common challenges and solutions

Webinar

Comparability Studies: Minimize Time, Maintain Quality

Developing Methods for Comparability Studies of Therapeutic Monoclonal Antibodies

White Papers (8)

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White Paper

White Paper: Facilitating Analytical Characterization of Biosimilars D...

Orthogonal Analysis Using Ligand Binding Kinetics and Live Cell Binding

Key Considerations for Automating Octet® Assays: Ligand Screening at Avitide
White Paper

Automating Octet® Assays for Ligand Screening

Drug development and production is challenging. Avitide, based in Lebanon, New Hampshire, provides on-demand development and supply of high-performanc...

End of End-Point Assays cover
White Paper

The End of End-Point Assays

Faster time-to results allows assessment of accurate and precise data earlier in the workflow

Investigating Synthetic Immune Recruitment by Proximity Inducing Molecules - White Paper
White Paper

Investigating Synthetic Immune Recruitment

by Proximity Inducing Molecules

White Paper

Streamlining Affinity Analysis for Accelerated Lead Screening

Antibody and other protein therapeutics are a major focus in drug discovery pipelines today

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White Paper

Writing Grant Proposals for Biomolecular Interactions Research

Tips collected over the years from customers successful with their equipment grant proposals.

Enhanced Efficiency in Lot Release and In-Process Testing of Biologics cover
White Paper

Enhanced Productivity and Labor Efficiency

In Lot Release and In-Process Testing of Biologics in GxP Laboratories

Octet Instrument Comparability Kinetics Precision Assessment
White Paper

Instrument Comparability Assessment: Kinetics Precision Assessment in...

PDF 1.3 MB
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Biolayer Interferometry (BLI)

Experience the difference of label-free Octet® BLI Systems

Octet® Publication Database

Literature references covering a large variety of research areas and applications

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