Specialty Media for HEK293 Cell Lines
Growth in gene therapies and viral vector vaccines has increased the demand for robust HEK293 cell lines. Sartorius offers four high-performing suspension HEK media that are designed for use with the high variability of HEK293 cell lines, the versatility of adeno-associated virus and the complexity of viral vector processes. The portfolio also includes a unique feed supplement to boost viral production for gene therapies and vaccines.
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HEK-FS_2 is designed as a feeding solution in combination with Sartorius HEK293 media. The feed contains highly concentrated nutrients to increase the productivity of HEK293 and other human cells.
Chemically defined, animal component-free cell culture supplement
Developed to feed HEK media portfolio
Contains no L-glutamine, serum, hydrolysates or growth factors
Supports superior production of AAV or LV in suspension cultures by maintaining and extending the production ability of HEK cultures
To be used in a pH-controlled environment, must be added 6 – 24 h after transfection, at a minimum.
Improve process economics in cell and gene therapies.
We talk to our media experts about the challenges in gene therapies and gene-modified cell therapies and how your HEK293 media can help.
Discover the key to efficient AAV production and significantly improve your production outcomes.
Understand and overcome challenges in AAV production and characterization processes and develop strategies for success
A checklist from research and development to clinical and commercial production.
Advances in cell culture have benefits throughout manufacturing. See how new developments can help optimize HEK cell line performance.
In this article, our expert shows you how to find the best HEK293 media for your gene therapy or viral vector vaccines process.
In this eBook, our experts reveal valuable insights to help you develop an optimal cell culture media strategy for your bioprocess.
Learn about the application of design of experiments (DOE) in combination with automated multi-way bioreactors (Ambr® 15) to expedite LV development
Secure your product’s stability and integrity during long-term storage and shipping
High-quality solutions designed and validated for the cryopreservation and cold storage of cells, including stem cells, T cells, and PBMCs.
Sartorius offers a complete scalable bioreactor platform from cell and strain line development to commercial manufacturing.
Perform label-free, rapid viral quantitation for gene therapy applications.
Sartorius’ global manufacturing facilities fully meet the highest quality standards and consistently supply powder and liquid buffers
Quickly and precisely quantify total virus particles to get real-time insights into viral titers.
Viruses are biological entities that replicate only within living cells. Most viruses can be grown in cultured cells such as MDCK, Vero, HEK, or insect cells. Historically embryonated hen's eggs were used to produce viruses before the industry moved to cell culture models.
The cell culture medium used to culture a virus greatly depends on the requirements of the host cell line. Specialty media provide the optimum quantity of nutrients to allow for cell growth and support virus infection and production. Among the human cell lines, HEK293 is widely utilized in viral vector production due to its high transfectivity, rapid growth rate, and ability to grow in a serum-free, suspension culture.
HEK293 cells are easy to grow and maintain in culture. They are also highly amenable to transfection, delivering excellent transfection efficiencies. Thus, HEK293 cells are a preferred cell line for transfection experiments to produce gene therapies or gene-modified cell therapies.
Specialty media designed for HEK293 cells provides the optimal quantities of necessary nutrients to allow maximum cell growth, enable transfection, and support the production of high viral titers. Ideally, cells should be cultured in chemically-defined, serum-free media to limit variability between batches and simplify regulatory approvals.
4Cell® NutriVero™ Flex 10 is a chemically defined, serum-free, animal component-free medium, designed to support Vero cell growth.
4Cell® Insect CD Medium is chemically-defined and designed to support the growth of insect cell lines derived from Spodoptera frugiperda.
Automated microbioreactor system for cell line development and process optimization, with 24 or 48 single-use 10-15mL microbioreactors.
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