High-Throughput Cytometry Resources | Sartorius

iQue® High-Throughput Screening (HTS) Cytometry

Access application notes, white papers, webinars, eBooks, guides, testimonials, videos, case studies and other resources, to aid your HTS Cytometry research  

Find your appropriate application needs, in the use of the iQue® High-Throughput Screening (HTS) by Cytometry Platform, together with validated iQue® reagents and time-saving iQue Forecyt® software usage. Discover how your peers are using High-Throughput Screening cytometry with the resources listed below.

Resources are available in these application areas:

Featured Flow Cytometry Resources

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Monitor and characterize iPSC culture and differentiation podcast header
Podcast

Poster Presentation Snapshots: Monitor and Characterize iPSC Culture

iQue® Podcast: Monitor and characterize iPSC culture and differentiation using Cytometry and Live-Cell Analysis.

eBook

First Edition High-Throughput Screening Cytometry Handbook

A Guide to HTS Cytometry Assays and Workflows

Application Note

A Novel Approach to Evaluating NK Cell Killing

Antibody Characterization 

Application Notes

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Application Note

Comparison of Anti-TNFα Monoclonal Antibody Biosimilars

Combining Binding and Functional Analyses for Comparison, of Anti-TNFα Monoclonal Antibody Biosimilars

Application Note

Quantification of Antibody-Dependent Cellular Phagocytosis

The iQue® Human Antibody Dependent Cellular Phagocytosis Kit plus the iQue® 3 provide a simple, robust assay for measuring ADCP.

eBooks

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Accelerating Drug Discovery using Systematic Cell and Protein Analysis
eBook

Accelerating Drug Discovery through Systematic  Cell and Protein...

This eBook presents the unique benefits of flow cytometry.

eBook

Antibody Screening Solutions Made Easy eBook

Beyond Monoclonal Antibodies - Exploring the Next Generation of Antibody Therapeutics
eBook

Beyond Monoclonal Antibodies

Exploring the next generation of antibody therapeutics, such as antibody–drug conjugates (ADCs), bispecific antibodies and nanobodies.

Publications

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Cytometry Applications

Cytometry Applications for Antibody, Small Molecule and Phenotypic Screening

Flow Cytometry for Antibody Discovery, Engineering and Development

Highlighting the iQue® Platform in antibody discovery and development for infectious diseases, neurological disorders, cancer and more.

Videos

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Video

Video and Poster: T Cell Characterization in 3D Cell Models using Adva...

This video and poster describe use of the iQue® Advanced Flow Cytometry Platform, to ascertain T cell phenotype and function in a physiologically rele...

Webinars & Videos

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Webinar

Antibody-Drug Conjugates (ADCs) Webinar

Simplifying the Characterization of the drug trastuzumab to Kadcyla®️ and Enhertu®️

Webinar

Simplified Characterization of Cells

Evaluating Cell Count, Health and Intracellular Marker Expression Using Advanced Flow Cytometry

Webinar

Evaluating Therapeutic Antibodies

Quantification of ADCP using Advanced Flow Cytometry

Webinar

Accelerating Antibody Characterization through Flow Cytometry Webinar

White Papers

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White Paper

White Paper: Facilitating Analytical Characterization of Biosimilars D...

Orthogonal Analysis Using Ligand Binding Kinetics and Live Cell Binding

White Paper

Publications Review

Advanced Flow Cytometry Applications for Antibody, Small Molecule and Phenotypic Screening

White Paper

Understanding T Cell Phenotype and Function to Enable Improved Therape...

T cells play a vital role in pathogen elimination and tumor immunosurveillance.

White Paper

Antibody Screening White Paper

Cell Health & Proliferation

Application Notes

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Application Note

Evaluating Antibody Drug Conjugates (ADCs) with 3D Tumor Spheroids

A combined workflow approach of iQue® High-Throughput Screening (HTS) by Cytometry and Incucyte® Live-Cell Analysis.

Application Note

Use of iQue® Proliferation and Encoding Dyes for Cellular Assays

This application note exemplifies the wide range of applications for the iQue® Cell Proliferation and Encoding Dyes.

Induced Pluripotent Stem Cells iPSC Cell Therapy
Application Note

Optimizing iPSC Culture Protocol with Growth Factors & Cytokines

Effectively use growth factors & cytokines with iPSC media to preserve pluripotency, support growth & increase time between feedings.

Solutions for Culturing, Maintaining and Characterizing iPSCs
Application Note

Solutions for Culturing, Maintaining and Characterizing iPSCs

Learn about an accelerated and streamlined workflow approach for the culture, monitoring and characterization of iPSCs.

Quantifying T Cell Response in 3D Tumor Spheroids Using Advanced Flow Cytometry Workflows
Application Note

Quantifying T Cell Response in 3D Tumor Spheroids

Two High-Throughput Screening by Cytometry solutions to address problems with traditional methods for measuring T cell response in 3D models

utilizing advanced flow cytometry and live-cell imaging to evaluate iPSC pluripotency During Cell Line Selection
Application Note

iPSC Pluripotency During Cell Line Selection and Differentiation

Combined iQue® 3 and Incucyte® live-cell analysis workflow approach for evaluating iPSC pluripotency and differentiation.

characterization and optimization of induced pluripotent stem cell culture using advanced flow cytometry and live-cell analysis
Application Note

Selecting Optimal iPSCs Using a Combined Workflow

Understand methods for the characterization, maintenance, scale up, and selection of ideal iPSCs in a combined workflow approach

Application Note

iQue® Human 4-Plex Apoptosis Kit Using Advanced High Throughput Flow C...

This application note illustrates the use of the  iQue® 3 advanced flow cytometry platform and iQue® Human 4-Plex Apoptosis Kit to measure apopto...

Articles

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Addressing High Throughput and High Content Challenges in Drug Discove...

eBooks

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eBook

eBook: Advances in Induced Pluripotent Stem Cell Research

Utilizing High-Throughput Screening (HTS) by Cytometry Based Techniques, in iPSC Culture, Characterization, and Optimization.

eBook

Article Collection: Advanced Flow Cytometry for Stem Cells

Role of cytometry in production, characterization and expansion of induced pluripotent stem cells for clinical applications.

Infographics

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Infographic

Infographic: An Approach To Assessing Cell Health And Viability

Proliferation, apoptosis, and cytotoxicity are measurements for study of the effects of drugs, culture conditions and genetic modifications.

Posters

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Small Molecule SAR Library Poster

Technology Brief

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Cy-Clone Plus Technology Brief

Webinars & Videos

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Antibody drug conjugate with drug compounds linked to IgG
Webinar

On-Demand Webinar: High-Throughput Assessment of ADC Cytotoxicity

Targeted Tumor Destruction: High-Throughput Assessment of ADC Cytotoxicity using High Throughput Cytometry

Illustration of the destruction of a human cell by lysis- shows apoptosis and mitochrondria

Exploiting the Mitochondrial Pathway of Apoptosis

Understanding the intrinsic, or mitochondrial, pathway of apoptosis has produced potent targeted therapies such as venetoclax.

On Demand Webinar: Evolution of Flow Cytometry in Infectious Disease R...

This panel discussion focuses on the use of flow cytometry, within four pathogenesis aspects, of the human body response to infectious diseases

Critical Insights for Drug Discovery Webinar

SLAS 2020 Tutorial SAR Compound Library Screen Webinar

Use of the iQue as a Versatile Tool for In Vitro Immunogenicity and Im...

Videos

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Monitor and characterize iPSC culture and differentiation podcast header
Podcast

Poster Presentation Snapshots: Monitor and Characterize iPSC Culture

iQue® Podcast: Monitor and characterize iPSC culture and differentiation using Cytometry and Live-Cell Analysis.

Video

iQue Forecyt® Software: EC50/IC50 curves and iQue® Human NK Cell Killi...

This video sequence reveals the ease of creation and review, of dose responses using iQue Forecyt® Software and data from the iQue® Human NK Cell Kill...

White Papers

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White Paper

Advanced Flow Cytometry – Rapid Analysis for Complex Cell-Based Models

White Paper on high throughput screening assays commonly required for early-stage candidate identification modern drug discovery pipelines.

Small Molecule Next-Gen Phenotypic White Paper

Immune Cell Function

Application Notes

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Application Note

Characterization of T Cell Activation and Function in Cancer Therapy

High-Throughput Screening (HTS) by Cytometry’s use in Cancer Immunotherapy, with the potential to predict on-target off-tumor effects.

Application Note

Comparison of Anti-TNFα Monoclonal Antibody Biosimilars

Combining Binding and Functional Analyses for Comparison, of Anti-TNFα Monoclonal Antibody Biosimilars

Application Note

High-Throughput Cytometry to Quantify Live Cell Antibody Binding

Quantify Direct and Competitive Live Cell Antibody Binding, to bring speed and hit quality to antibody discovery.

Multiplexed SARS-CoV-2 Serological Assay Using Advanced Flow Cytometry
Application Note

Multiplexed SARS-CoV-2 Serological Assay

In this study, use of the iQue® SARS-CoV-2 Kit allowed for rapid, simultaneous detection of all three major isotypes of antibodies specific for SARS-C...

Complement-Dependent Cytotoxicity with Miniaturized Advanced Flow Cytometry
Application Note

Complement-Dependent Cytotoxicity with Miniaturized Advanced Flow Cyto...

Learn about a streamlined, miniaturized workflow for quantifying mAb induction of Complement-Dependent Cytotoxicity using the iQue®.

Application Note

Beyond Monoclonal Antibodies

Exploring the next generation of antibody therapeutics

cytokine app note
Application Note

Combining Live-Cell Analysis and High-Throughput Flow Cytometry

To Gain Additional Insights into the Mechanisms of Immune Cell Killing of Tumor Cells

Application Note

iQue® Mouse IgG Type and Titer Assay Kit

 A Novel Solution to Expedite Antibody Discovery

Multiplexed SARS-CoV-2 Serological Assay Using Advanced Flow Cytometry
Application Note

Multiplexed SARS-CoV-2 Serological Assay

In this study, use of the iQue® SARS-CoV-2 Kit allowed for rapid, simultaneous detection of all three major isotypes of antibodies specific for SARS-C...

Application Note

A Novel Approach to Evaluating NK Cell Killing

Quantifying T Cell Response in 3D Tumor Spheroids Using Advanced Flow Cytometry Workflows
Application Note

Quantifying T Cell Response in 3D Tumor Spheroids

Using Advanced Flow Cytometry Workflows

Application Note

Quantitative Analysis of CAR-T Cell Exhaustion

Quantitative analysis of CAR-T Cell Exhaustion using the iQue® Advanced Flow Cytometry Platform and the iQue® Human T Cell Exhaustion Kit.

Application Note

Utilizing MLR to Evaluate Checkpoint Inhibitor Therapies

Using Advanced Flow Cytometry

Case Studies

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Case Study

Trust in Your Results — Experience the Benefits of iQue® Advanced Flow...

eBooks

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Beyond Monoclonal Antibodies - Exploring the Next Generation of Antibody Therapeutics
eBook

Beyond Monoclonal Antibodies

Exploring the next generation of antibody therapeutics, such as antibody–drug conjugates (ADCs), bispecific antibodies and nanobodies.

eBook

Functional Profiling of Immuno and Cell-Based Therapies

The articles in this eBook provide a taste for some I-O toolbox essentials with the iQue® Advanced Flow Cytometry Platform and associated reagent kits...

Advanced Flow Cytometry: Advancing the Development of Immunotherapies
eBook

Advanced Flow Cytometry in Immuno-oncology

In this article collection, we will examine how advanced flow cytometry can further the development of immunotherapies.

eBook

Phenotypic Screening Advances

Download this eBook to learn more about the benefits of phenotypic screening, as well as some of the most burdensome challenges and the novel solution...

eBook

Deciphering Immune Response to Viral Infection

This GEN eBook features a collection of articles and studies that focus on efforts to unravel the complexity of viral biology and host immune response...

eBook

Advances in Immune Cell Profiling

Assessing T cell populations at the tumor core and margins by identifying biomarkers may be more effective when deciding on patient prognosis than the...

eBook

Ebb and Flow: Investigating Immune Response

Using Advanced Flow Cytometry

immuno-oncology ebook cover
eBook

Immuno-oncology: Understanding Cell-Based Therapeutics

eBook

Multiplexing Phenotype & Function for More Relevant Insights

eBook

Novel Trends in Immuno-Oncology Research

Guides & Publication Reviews

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Publications

Advanced Flow Applications in Infectious Disease & Immuno-oncology

Posters

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Poster

T-Cell Activation Poster

This poster describes the development of a large-scale multiplexed assay using high throughput flow cytometry to profile T cell activation.

Poster

iQue® Application Posters: Antibody Discovery and Immuno-Oncology

Poster

Immuno-Oncology T-Cell Activation Poster

This poster highlights the development of a large-scale, multiplexed assay using the Intellicyt® iQue Screener PLUS platform to assess T-cell activati...

Poster

ADCC Poster

Rapid Multiplexed Profiling of Antibody Dependent Cell Cytotoxicity

Supplements

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Cell Press Engineering Immune Cells for Cancer Therapy Supplement

The reviews and research in this Cell Press supplement offer a snapshot of the latest advances in this rapidly evolving field.

Cell Press New Approaches to Drug Screening Supplement

Webinars & Videos

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Webinar

Go with the Flow: Advancing Cell and Gene Therapy

Discussion on advances in gene and cell therapy fields, and the technologies which are progressing the next generation of these treatments.

Webinar

CAR-T: Why Not Me?

Do we have an idea of which patient factors determine the success of CAR-T therapies?

Video

iQue® Poster Presentation Snapshots

Quantifying in vitro Complement-Dependent Cytotoxicity (CDC) using Advanced Flow Cytometry

Webinar

Accelerating Antibody Discovery Using Advanced Flow Cytometry

This webinar discusses the use of advanced flow cytometry to assess antibody/cell interactions in the context of live cell antibody binding, competiti...

Webinar

 Immunological Responses to Stroke

Advanced flow cytometry, which combines high-throughput sampling and advanced data analytics, enables rapid cell phenotyping and cytokine quantitation...

Webinar

Importance of Immune Profiling in CAR-T Therapies live

This webinar explores the power of flow cytometry in this growing research area.

Webinar

A New Multiplexed SARS-CoV-2 Serological Assay

Using Advanced Flow Cytometry

Webinar

Storm Chasing: Cytokine Profiling Flow Cytometry Age-Related...

This webinar details neuroimmune interactions, cells models for the study of disease and also possibilities of the development of therapeutics, which...

iQue Screener PLUS
Webinar

Measuring T Cell Exhaustion: A Multiplexed Approach Utilizing Advanced...

Advanced flow cytometry merges high-throughput sampling and advanced data analytics, to improve the identification and analysis of complex biological...

Video

iQue Forecyt® Software and T-Cell Activation Workflow

This video sequence demonstrates the ease of rapid, high content data generation. Faster and more robust, trend and hit identification, are also shown...

Video

Poster Presentation Video: Donor and Antibody Diversity in NK Cell-Med...

This presentation describes key advantages of the iQue® Advanced Flow Cytometry Platform, iQue® Human NK Cell Killing Kit and iQue® Human NK Cell Comp...

Webinar

On-Demand Webinar: Evaluation of Checkpoint Inhibitor Therapies using...

Webinar

Cell-Based Therapies for Cancer Treatment: Leveraging Advanced Flow Cy...

Webinar

Next Generation Phenotypic Screening

This recorded webinar highlights how AstraZeneca leveraged the unique capabilities of the Intellicyt iQue® Screener platform to run a 500 thousand com...

Webinar

Gaining Comprehensive Insights from T-Cell Biology Using Advanced Flow...

Discussion profiling T Cell memory subsets and functional cytokine release, using a rapid a multiparametric assay.

Webinar

Evaluating Immunotherapy Webinar

Evaluating Immunotherapeutic Approaches with Large Scale Functional Assays for Immuno-Oncology

Webinar

A Novel Immuno-Oncology Screening Platform for Multifactorial Evaluati...

This on-demand webinar highlights how researchers at Horizon Discovery leveraged the iQue® advanced flow cytometry platform to deliver rich datasets f...

campaign image for webinar Deriving context from immunological data with advanced flow cytometry
Webinar

Deriving Context from Immunological Data

Webinar

Evaluation of Checkpoint Inhibitor Therapies using an MLR Assay

Webinar

Evolution of Flow Cytometry in Infectious Disease Research

Webinar

Harnessing the Immune System to Fight Cancer

Webinar

Multiplexed Functional Antibody Profiling

Using Advanced Flow Cytometry

Webinar

A High Throughput Approach to Developing T-Cell Immunotherapies Webina...

Webinar

CAR-T Cell Therapy Bispecific Antibodies Webinar

Webinar

New Horizons in Therapeutic Antibody Discovery: Challenges and Opportu...

Webinar

Dismantling SARS-CoV-2, One Analysis at a Time Webinar

Webinar

Utilizing Synthetic DNA Technologies

For High Throughput Antibody Discovery and Optimization

iQue Poster Presentation Snapshots:

Assessment of NK Cell-Mediated Killing and Phenotypic Analysis

iQue Poster Presentation Snapshots:

A High Throughput, Multiplex Antibody Internalization Assay

White Papers

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White Paper

Publications Review

Advanced Flow Cytometry Applications in Infectious Disease and Immuno-oncology

White Paper

Multi-Parametric Analysis Antibody Discovery Workflows

White Paper:  High Throughput, Multi-Parametric Analysis Accelerates Antibody Discovery Workflows

White Paper

Ebb and Flow: Investigating Immune Response

This eBook describes some of the challenges that researches face when characterizing the immune response, and how advanced flow cytometry solutions ar...

White Paper

CAR-T Therapies Solid Tumors White Paper

Read the interview by Dr. Tamara Laskowski titled ‘Unlocking the Potential of CAR-T Therapies for Solid Tumors’ in which she describes the progress re...

Phenotypic and Functional Characterization of CAR-T Cells white paper
White Paper

Phenotypic and Functional Characterization of CAR-T Cells with Advance...

This white paper introduces the process of manufacturing and expanding cell therapy products, with a focus on CAR-T cells.

White Paper

Understanding T-Cell Phenotype and Function

To Enable Improved Therapeutics

Use of Advanced Flow Cytometry to Accelerate Antibody Screening and Characterization, and Reveal Deeper Biological Insights cove
White Paper

Use of Advanced Flow Cytometry to Accelerate Antibody Screening and Ch...

White Paper

Addressing the Challenges in Biologics Discovery with Advanced Flow Cy...

Explore iQue® HTS Cytometry Products & Applications

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High-Throughput Screening Cytometry Products

The iQue® HTS Cytometer allows researchers to acquire and analyze high content, multiplexed assays needed to assess immune cell function

High-Throughput Screening Cytometry Applications

A faster, smarter cytometry platform enables multiplexing cell phenotypthate and function to get you from samples to actionable results faster

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