Ready, Set, Flow: Intuitive and Effortless Crossflow for the Lab
Also known as crossflow filtration, TFF is used to clarify process fluids such as cell culture media, and to concentrate and buffer exchange macromolecules such as proteins, viruses, nucleic acids, and nanoparticles. Although TFF originated in commercial manufacturing, it is increasingly relevant and popular in research laboratories where larger sample (feed) volumes need to be processed.
Groundbreaking Vivaflow® cassettes from Sartorius simplify TFF for researchers who need to prioritize efficiency over complexity. Available in single use and reusable formats with a comprehensive selection of membrane materials and MWCOs, you can always find the right solution for effortless concentration and buffer exchange from 0.1 to 5 L feed volumes.
Plug and play TFF cassettes with no need for cleaning featuring 50 cm² PES membranes
Budget-friendly TFF cassettes that can be re-used featuring 50 cm² Hydrosart® RC membranes
Economical TFF cassettes for multiple UF | DF runs featuring 200 cm² PES or Hydrosart® RC membranes
Precise flow control for Vivaflow® and other fluid transfer applications
Ready to go with the flow. Effortlessly scale up from centrifugal ultrafilters to TFF cassettes, with fast and simple set up right out of the box.
Push your research budget further. Avoid expensive TFF systems and cassette holders with an all-in-one design that is built to run with a standard peristaltic pump.
Put down the calculator. Advanced flow paths and a fixed occlusion restrictor deliver ultra-efficient TFF without tedious process optimization.
Lossless handling and ultimate flexibility. Maximize capacity, speed and recovery for virtually any molecule, with modular single-use or reusable options to suit every process.
In TFF, a sample flows parallel to the membrane surface in a cassette. A pump provides positive pressure to recirculate the sample between the cassette and a feed reservoir and drive the continuous separation of particles or macromolecules (feed | retentate) from the liquid (permeate) based on size or molecular weight. The constant flow of feed | retentate also generates low shear stresses at the membrane surface to effectively prevent fouling, polarization and blocking.
Need to clarify a secreted protein or virus before TFF?
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TFF is an efficient method for concentration and buffer exchange from feed volumes ≥100 mL.
When feed volumes exceed the capacity of centrifugal ultrafilters, concentration and buffer exchange can become labour intensive and time consuming. However, TFF based on scale-down process technology is expensive and complicated.
Vivaflow® is the only TFF solution designed specifically for the lab. Its unique design allows for fast and intuitive set up and efficient UF | DF with only a peristaltic pump, eliminating the need to optimize feed flow rates, transmembrane pressures, and filtrate flux. This substantially reduces cost and user effort while ensuring high process speed and maximum target recovery.
Scale-Down TFF Cassettes
Vivaflow® TFF Cassettes
Economical
Easy Set Up
Optimization-Free
Easy for you and gentle on your sample. The Vivaflow® feed reservoir makes both ultrafiltration and diafiltration extremely convenient. First, a sample is concentrated to the desired final volume. Then a length of tubing is placed into a separate vessel containing the exchange buffer and connected to the reservoir. An airtight seal in the feed reservoir lid allows continuous diafiltration - as the original buffer continues to permeate the ultrafiltration membrane, it is replaced by an equivalent volume of exchange buffer. This reduces buffer consumption and avoids sample dilution.
Explore Diafiltration
Continuous diafiltration is quick and easy with the Vivaflow® feed reservoir.
The modular design of Vivaflow® allows multiple cassettes to be connected in series or parallel. This supports increased throughput for larger feed volumes or increased processing speed for smaller samples. To connect two Vivaflow® 50 cassettes, simply use the serial connector included in the box. To connect Vivaflow® 50R | 200 cassettes in parallel, T and Y connectors can be ordered as optional accessories.
Run multiple Vivaflow® cassettes together, to increase throughput or reduce time-to-result.
Discover the complete and comprehensive range of Sartorius laboratory ultrafilters.
Find the technical specifications and ordering information for the original lab TFF cassettes.
Choose the right pump for your ultrafiltration and diafiltration processes with Vivaflow®.
Vivaflow® TFF cassettes are available with the following MWCO | pore size membranes:
For more detailed information on membrane availability, please refer to the product datasheets.
Two cassettes, two tubing kits (for individual operation), one serial connector (for serial operation), and an instruction manual.
One cassette, one tubing kit (for individual operation), one pressure gauge, and a quick start guide.
Yes, they are. While we have taken steps to minimize our environmental impact with quick start guides in the product packaging, if you need more information on how to use our products, the full instruction manual can be downloaded from this product page or in the eShop.
Cassettes with a 50 cm² membrane area are recommended for feed volumes of 0.1 - 0.5 L. Two cassettes can be run in series or parallel (depending on the chosen model) to double the throughput to 1 L.
For feed volumes from 0.5 - 2.5 L, cassettes with a 200 cm² membrane area are recommended, and throughput can again be doubled, up to 5 L, by running two cassettes in parallel.
A peristaltic pump is all that is needed to use Vivaflow® cassettes. Sartorius recommends and sells Watson-Marlow pumps for this purpose.
The optional stand is a useful accessory for Vivaflow® 50 cassettes to keep your workspace tidy, and we recommend using a pressure gauge that is available to order separately for Vivaflow® 50, and included in the pack with Vivaflow® 50R and Vivaflow® 200 cassettes.
You can use any suitable bottles, beakers, or other vessels to contain your feed sample and collect the permeate. However, we recommend using the dedicated feed reservoir for diafiltration.
Most configurations require the pump head for 1.6 mm WT tubing. The pump head for 2.4 mm WT tubing is only needed when you will run two Vivaflow® 200 cassettes in parallel.
A pack of T-connectors is required to run two Vivaflow® 50R cassettes in parallel. One T-connector is used to divide the feed stream between the two cassettes, and the other to combine the retentate streams for return to the feed reservoir.
Vivaflow® 200 requires one Y-connector and one pack of T-connectors. The Y-connector replaces the standard feed tubing and is used to divide the feed stream between the two cassettes. A T-connector is used to combine the retentate streams for return to the feed reservoir.
These TFF cassettes are stable for use with most common aqueous buffers and some solvents such a 70% ethanol. Additional compatibility is afforded by the reusable cassettes. For further information, please refer to the instruction manuals.
For laboratory dedicated TFF, Vivaflow® offers a wide choice of membrane materials and MWCOs. This already helps to ensure high recoveries of most molecules.
In addition, after UF | DF, a common process step in TFF is to perform a low volume buffer rinse through the tubing and cassette(s). The resulting sample will recover residual molecules that can be combined with the retentate to typically collect ≥98% of the starting sample quantity.
No. Vivaflow® 50 is intended for single use and is not compatible with typical cleaning agents.
Reusability can vary from sample to sample, but these cassettes only need to be used about three times to benefit from low operating costs.
We recommend replacing Vivaflow® 50R and Vivaflow® 200 cassettes after a maximum of five runs, and always before the expiry date printed on the packaging label. Separate cassettes should be used for each target molecule or sample batch to eliminate the risk of carryover.
Wear to peristaltic pump tubing is expected with normal use. Therefore, we recommend replacing the supplied tubing with a new tubing kit for the second and any subsequent runs.
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