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Sartoclear Dynamics®️ Lab
One-Step Clarification and Sterile Filtration of Cell Cultures
Sartoclear Dynamics® Lab kits are designed for rapid harvesting 15 mL to 1,000 mL volumes of cell cultures in the lab, enabling clarification and sterile filtration to be performed in one step. These kits simplify the process by fully eliminating the centrifugation step otherwise needed for clarification. As a result, up to 1,000 mL cell cultures can be efficiently clarified and sterilized in minutes – quickly and easily.
This video provides a detailed walkthrough of the purification process, starting from cell lysis all the way to obtaining a concentrated retentate. It highlights the use of a clarification step employing Sartoclear Dynamics®️ Lab to eliminate larger contaminants such as cells and cell debris. Following this, an ultrafiltration step using Vivaflow®️ is demonstrated to concentrate the AAV particles and further cleanse the preparation of smaller impurities.
Join us to explore the intricacies of AAV purification and enhance your understanding of this vital component of gene therapy development.
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Sartoclear Dynamics Lab clarifies high-density mammalian cell cultures faster and more efficiently than other processes.Watch this comparison video.
Clarification and sterile filtration of up to 1 L mammalian cell culture combining a filter aid for clarification and a vacuum filtration unit for sterile filtration.
For simultaneous filtration of up to 6 x 50 mL samples without the need for installation of extra connectors and time-consuming stabilization of filter units.
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Convenient, ready-to-use-kit combines a 20 mL syringe pre-filled with a filter aid for clarification with a 0.2 um polyethersulfone filter for sterile filtration.
Get answers to any questions you have about Sartorius cell harvesting devices.
The team at LifeArc, a medical research charity based in the UK, is using the Sartoclear Dynamics®️ Lab and Sartolab®️ Multistation to clarify high-density cell culture media in minutes, creating a workflow that’s more efficient and productive.
"Processing mammalian cell culture fluid can be particularly challenging, as in the lab we are often driven by the need to perform this task quickly with minimum loss in the quantity of cell culture fluid obtained. Standard vacuum filtration isn’t always the ideal solution due to limitations in the sophistication of the equipment itself. It often requires additional supervision and can be quite time-consuming to undertake, particularly when there are several samples involved.
The Multistation has significantly helped our lab. In addition to the six-sample capacity, the independently controlled multi-port design has provided operators with the flexibility to perform additional tasks in parallel, thereby delivering greater efficiency overall in the lab with improved cell culture fluid harvesting."
- Richard Brown, BSc, Scientist at LifeArc
"As a leading viral-vector manufacturer, we strive to produce the highest quality rAAVs in the shortest possible time. Isolating rAAVs from crude cell-lysates typically requires centrifugation and/or PEG precipitation – steps which are time consuming, labor intensive and generate a lot of plastic waste.
The single-use Sartoclear Dynamics® Lab Filter kits has enabled us to streamline our upstream processes. The crude-lysates are directly filtered over Sartoclear Dynamics® Lab Filter kits. Clarification is achieved within a few minutes and the Multistation set-up allows us to simultaneously filter up to 6 L. The rAAV-recoveries using Sartoclear Dynamics® Lab products are remarkably high, surpassing what we obtain using in-depth filtration. Moreover, lysates clarified using Sartoclear Dynamics® Lab are instantly ready for our downstream processes including affinity capture by FPLC. Sartoclear Dynamics® Lab, along with the Multistation, have greatly improved our workflow."
— Kimchi Strasser, PhD., Team Lead for Process Development at Sirion Biotech GmbH (a Perkin Elmer company)
Discover how the Sartoclear Dynamics® Lab filtration kits can dramatically reduce filtration times for high-density bacterial supernatants, streamlini...
Sartoclear Dynamics®️ Lab protocol for the production of large volumes of cell-free bacterial isolates without centrifugation
In this study, the Sartoclear Dynamics® Lab V500 Kit was tested as a novel method for the clarification of cell culture media prior to purification of...
This study evaluates a novel method for lab-scale clarification of mammalian cell culture supernatants.
The novel Sartoclear Dynamics® Lab V Kit was evaluated for the removal of transiently IgG expressing mammalian MEXi-293E (HEK293) cells from cell...
In this study, we describe our tests of Sartoclear Dynamics® Lab V, a novel system for rapid clarification of cell culture media without the need...
Clarification of 50 mL suspension adapted HEK-293-6E cells, transiently expressing human IgG4 by comparing a conventional harvest method and the Sarto...
In this study, the use of Sartoclear Dynamics®️ Lab V50 comprising the diatomaceous earth filter aid was evaluated for clarification of lentiviral vec...
This study aimed to evaluate the applicability of Sartoclear Dynamics® Lab Filter Aid.
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