Solutions to Expedite and Simplify iPSC Characterization
Understanding the complexities of induced pluripotent stem cells (iPSCs) and defining the differentiation protocols needed to deliver the desired cell and tissue types requires:
Sartorius' solutions for functional and phenotypic characterization of iPSCs enables the discovery and multiplexed screening validation of differentiation protocols, allowing you to accelerate the transfer to process development and time to market.
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Formulate and freeze aliquot supplies into cryocontainers.
Detailed and comprehensive characterization methods are needed to ensure patient safety including biosafety testing, genetic stability, pluripotency phenotype as well as cell viability and more.
Gently obtain final iPSC products with maintained biological characteristics.
Expand iPSCs utilizing growth factors and cytokines to achieve production culture volume and cell density as well as to maintain cell functionality through the cryopreservation process.
Establishes stem cell source to be used for multiple products. Perform extensive qualification and characterization testing.
Select the best clones that meet defined quality criteria and ability to differentiate into desired cell type.
Expand clonal cells utilizing growth factors and cytokines to obtain sufficient supply for research and development needs – while constantly checking for consistent marker expression.
Genome editing might be necessary in some cases to influence the histocompatibility profile of stem cells.
Select target cells using pluripotency markers. Evaluate and compare individual iPSCs for pluripotency and differentiation with advanced techniques to ensure the best lines are selected moving forward.
Reprogramming of somatic cells with different methods, such us Lentivirus, Retrovirus, Sendai virus, Episomal vectors etc. Use growth factors and cytokines to influence specific behaviors in cells.
Sourcing representative cells for reprogramming.
Measuring the pluripotency and viability of iPSCs to aid selection of the most appropriate cell line to use for differentiation.
Apply protocols using specific agents such as transcription factors, cytokines, and growth factors to induce differentiation into target cells.
Check out this video to learn about the history and application of iPSCs as well as the critical steps in an iPSC R&D workflow.
Fully automated solution for the identification, targeted isolation and passaging of the desired stem cell colonies or clones.
See journal publication: Automated selection and harvesting of pluripotent stem cell colonies
Explore Clonal Stem Cell Picking
Enables real-time, live-cell imaging and analysis directly inside your incubator. It provides an easy way to check for pluripotency, confluency, and differentiation over time.
Explore Live Cell Assays
A high-throughput, cell and bead-based cytometry platform for the assessment of pluripotency and differentiation marker expression in combination with viability of iPSC cultures.
Miniaturized assay format due to the low volume requirements of the iQue® 3 reduces sample wastage, meaning more cells are available for downstream analysis, expansion, and differentiation
High-throughput sample acquisition (5 minutes for a full 96-well plate) reduces workflow time and facilitates enhanced replication, meaning more robust data
Plate-level, real-time data visualization tools enable rapid comparison between cell lines, resulting in the fastest path to actionable results
Explore High Throughput Cytometry
Explore our range of high-quality, animal-free research use only growth factors and cytokines for cell-based applications.
Explore RUO Cytokines
Octet®️ Label-free BLI analysis platform is a highly versatile tool that can be utilized to evaluate binding interactions across a variety of analytes ranging from small molecules, nucleic acids, proteins and peptides as well as nanoparticles.
Explore Gene Therapy Development
NutriStem® hPSC XF Medium is a powerful low-bFGF medium that works across research and clinical workflows, giving you ultimate control over your cultures.
Explore Media
CellCelector technology enables a gentle mechanical transfer method with high specificity maximizing cell viability and clonality of picked colonies while maintaining their pluripotent characteristics.
For picking of adherent colonies the CellCelector combines a very gentle, crosswise scrape movement with the simultaneous aspiration of the colony. This gently loosens the colony from the base of the culture plate or feeder cell layer.
Continuous morphological assessment for easy monitoring of iPSC colonies throughout differentiation with Incucyte® Live-Cell Analysis System.
Conserve precious sample and time during iPSC phenotyping with iQue® 3 High-Throughput Cytometry Platform by sampling as little as a single microliter from miniaturized assay volumes.
Effectively use growth factors & cytokines with iPSC media to preserve pluripotency, support growth & increase time between feedings.
Secure and configurable software module to track all system events and user actions.
Translational In Vitro Models for Studying Human Development & Disease
Quantitative live-cell analysis for optimization of culture conditions and evaluation of cell health in human iPSC-derived neurons.
Combined iQue® 3 and Incucyte® live-cell analysis workflow approach for evaluating iPSC pluripotency and differentiation.
Learn about an accelerated and streamlined workflow approach for the culture, monitoring and characterization of iPSCs.
With the iQue® 3 High-Throughput Cytometry Platform you can evaluate several iPSCs by measuring pluripotency marker expression and using the Incucyte® Live-Cell Analysis Platform you can observe the morphology of cells.
The CellCelector Flex, with its specifically designed scrape process for picking adherent cells and cell colonies, is extremely gentle, very specific and therefore ideally suited for the clonal passaging of stem cells, stem cell colonies as well as the isolation of specific parts of a stem cell colony.
The Octet® Bio-Layer Interferometry (BLI) platform with the newly developed AAVX Biosensor offer a quantitation dynamic range of 8.5x108 to 1.0x1013 vp/mL, high precision and broad AAV serotype binding specificity, allowing for the quantitation of 10 different serotypes.
The Incucyte® Organoid Culture QC Application is a turnkey solution consisting of a validated 3D cell culture protocol and software module for real-time visualization and label-free objective quantification to optimize organoid culture conditions.
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