Apoptosis Assays
Apoptosis is an essential process for normal tissue development and homeostasis by which cells undergo timely programmed cell death. Aberrations in apoptotic signaling are implicated in a range of human pathologies including cancer, autoimmune disease and neurodegeneration. Induction of apoptosis leads, in most cases, to the depolarization of mitochondria, the activation of caspases and plasma membrane alterations. The ability to simultaneously measure a multitude of apoptotic markers allows confirmation of pathway and insight into mechanism of action over time.
Request a Quote
Figure 1. Apoptotic markers measured by the iQue® Human 4-Plex Apoptosis Kit. 1) Mitochondrial depolarization, 2) Caspase 3/7, 3) Annexin V and 4) Non-viable. The kit components can be utilized either separately or combination in a no wash format. Apoptosis reagents can also be multiplexed with other iQue® HTS reagents in no wash assays.
Figure 2. Cytotoxic and cytostatic compounds produce different cell health profiles. Jurkats (1e6/mL) treated with a range of concentrations of 4 compounds for 24 hours. Before analysis with iQue®️ Human 4-Plex Apoptosis Kit.
(A) Caspase 3/7 expressing cells are shown in a heatmap from iQue Forecyt®️.
(B,C,D,E) Depolarized mitochondria, Caspase 3/7, Annexin V and Non-viable (respectively) cell percentages are shown.
Figure 3. Cell health markers show a time dependent increase when treated with an apoptosis inducing drug.Jurkats (1e6/mL) treated with a range of concentrations of Staurosporin. 20 µL samples were taken and analyzed after 2, 6 and 24 hours of treatment with 3 apoptosis reagents. (A) Heat map of Caspase positive cell (%) from 2 hours, (B) Mitochondrial depolarization, (C) Caspase and (D) Annexin V
Figure 4. Schematic of the simple, mix and read apoptosis assay.
Platform: Compatible with iQue®️ 3 BR/VBR & iQue®️ Plus BR/VBR
Catalog Numbers
1 x 384
90053
5 x 384
90054
20 x 384
90155
50 x 384
90156
Individual Apoptosis Reagents
Available Size
Catalog Number
B/Green
5 x 38420 x 38450 x 384
910349103591036
B/Yellow
910309103191032
R/Red
910389103991040
B/Red
903469034790348
Request a Demo
Combining Binding and Functional Analyses for Comparison, of Anti-TNFα Monoclonal Antibody Biosimilars
Understanding the intrinsic, or mitochondrial, pathway of apoptosis has produced potent targeted therapies such as venetoclax.
Perform comprehensive, multiplexed analysis of cells, beads and secreted proteins all from the same cells at the same timepoint.
iQue®️ Human 4-Plex Apoptosis Kit
A cytometry approach to assessing cell health and viability
A robust assay for measuring cellular progression through apoptosis via multiple pathways.
Development of a no-wash 4-plex apoptosis screening kit and validation for phenotypic screening
Information can be used by monitoring cell proliferation rates, optimizing growth conditions and normalizing cell data for further studies.
Determining cell viability is crucial when assessing a cell’s response to treatment in order to exclude them from final data analysis.
Please select your country so we can show you products that are available for you.
The content of our website is always available in English and partly in other languages. Choose your preferred language and we will show you the content in that language, if available.