Neurite Outgrowth
During development, neurons extend numerous processes that differentiate into dendrites and axons. These processes (termed neurites) are critical for communication between neurons. Characterization of neurite outgrowth, maturation, and the disruption of neurite networks is key to the study of neuropathological disorders (e.g. neurodegeneration), neuronal injury/regeneration and screening for neurotoxicity. However, current techniques used to study those dynamic biological changes are challenging.
With the IncuCyte® Live-Cell Analysis System and IncuCyte® NeuroTrack Software Module (Cat. No. 9600-0010) you can now achieve automated, continuous, non-invasive imaging and measurement of neurite outgrowth with 96-/384-well assays that generate meaningful, kinetic data with visual confirmation of biological changes.
Request More Information
See All Live-Cell Analysis Applications
Feature
Benefit
Simple, cell sparing protocols without fixing, staining, or antibody-labeling
IncuCyte® Neurite Analysis Assays enable automated, continuous analysis of neurite outgrowth using your choice of neurons (e.g. primary, iPSC-derived, immortalized neuronal cell lines) in monoculture or in co-culture with astrocytes. Quantify neurite length and branch points in order to evaluate neural network stability over time – all inside your cell culture incubator.
Monitor neurite outgrowth in live neuronal monocultures using IncuCyte NeuroTrack analysis software. Fully automated, long-term label-free analysis. Human iPSC-derived neurons (iCell Neurons, CDI) in monoculture.
Measure changes in neurite length and branch points in mixed cultures of neurons and astrocytes using fluorescent neuronal labelling reagents and IncuCyte analysis software. Primary rat forebrain neurons labeled with the IncuCyte® NeuroLight Orange reagent in co-culture with rat astrocytes.
View Incucyte® Neurite Analysis Publications
Visualize and quantify long-term changes in neurite outgrowth and disruption in real time in label-free monocultures or use IncuCyte® NeuroLight reagent or NeuroPrime Cell Kits for selective analysis of neurite dynamics in co-cultures.
Figure 1. Continuous measures of neurite dynamics.. IncuCyte NeuroTrack analysis software automatically analyzes images to provide measures of neurite length, branch points and neuronal cell body clusters.
Our turnkey solution enables automatic generation of time courses to reveal concentration-dependent responses with IncuCyte NeuroTrack’s unbiased, automated analysis in 96- or 384-well plates.
Figure 2. Measure treatment effects automatically and non-invasively. IncuCyte neurite analysis assays allow every well of a 96- or 384-well plate to be imaged and analyzed automatically to provide a microplate readout of neurite dynamics over time (A). Time-courses reveal concentration-dependent treatment effects (B). Transform data into concentration-response curves to compare pharmacology (C).
Make continuous measurements in relevant neuronal models in highly flexible, robust and reproducible assay formats suitable for screening and profiling. IncuCyte assays and reagents are compatible with iPSC-derived or primary cells, in mono- or co-culture.
Figure 3. Create relevant in vitro models of neurodegeneration and neurotoxicity — screen for neuroprotective treatments. Concentration dependent neurotoxic effects of 6-hydroxydopamine (6-OHDA) measured in human iPSC-derived dopaminergic neurons (Dopa.4U, Axiogenesis) in a functional model of Parkinson’s disease. Treatment with bone derived neural factor (BDNF) promotes neurite formation but provided little or no protection against the effects of 6-OHDA.
Real- time live cell protocols for continuous measurements of neuronal networks in monocultures or co-cultures using IncuCyte NeuroLight Lentiviral labeling reagents – no fixing, washing or labeling steps!
Neuronal monocultures—label-free protocol overview
Neuronal co-cultures—fluorescence protocol overview
Request More Information View Incucyte® Neurite Analysis Publications
Learn how IncuCyte live-cell analysis has been used by researchers around the world to drive neuroscience discovery.
Download Now
Product
Qty.
Cat. No.
Enables microplate analysis of neurite dynamics with or without labels
9600-0010
Live-cell neuronal labeling reagent for long-term expression of an orange fluorescent protein
4808
Live-cell neuronal labeling reagent for long-term expression of a red fluorescent protein
4807
Kit includes:
4760
4585
4753
4586
One vial of lyophilized reagent sufficient for 100-200 live-cell apoptosis tests in 96-well format
4642
4759
4641
4768
Effectively use growth factors & cytokines with iPSC media to preserve pluripotency, support growth & increase time between feedings.
Translational In Vitro Models for Studying Human Development & Disease
Incucyte® Reagents, Consumables and Software
For the Demonstration of Label-Free Neurite Outgrowth and Neuronal Cell Health
For Measuring Neurite Outgrowth
Kinetic, label-free assays for neurite outgrowth in primary, iPSC-derived and immortalized neurons
Please select your country so we can show you products that are available for you.
The content of our website is always available in English and partly in other languages. Choose your preferred language and we will show you the content in that language, if available.