Advanced Flow Cytometry and BLI Label-Free Detection for Binding and Functional Characterization of Anti-HER2 Antibodies

Binding and Functional Characterization of Anti-HER2 Antibodies

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Application Note: Characterization of Anti-HER2 Antibodies Using iQue® & Octet® Platforms

 Authors: Kirsty McBain, Daryl Cole, and Nicola Bevan | Last updated: 11/1/2022

Overview

For continued growth and diversification of the monoclonal antibody (mAb) therapeutic market we need robust, high-throughput techniques for screening and profiling drug candidates. Here we analyze anti-HER2 mAbs to demonstrate the power of a combined iQue® Advanced Flow Cytometry and Octet® BLI Label-Free Detection approach for antibody characterization. iQue® delivers rapid, live cell-based assessment of binding, competition and function, while Octet determines binding kinetics and evaluates post-translational modifications. 

  • Document type: Application Note
  • Page count: 1
  • Read time: 6 minutes

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Key Takeaways:

  • Discover a robust and high-throughput method for screening and characterizing mAb drug candidates
  • Identify mAbs that compete for epitope binding
  • Rank antibodies based on glycosylation status
  • Analyze Fc receptor function in immune cell co-culture
  • Assess binding and specificity on live cells

This Resource is Designed for:

  • Drug developers
  • Analytical scientists
  • R&D scientists

Applications Supported:

  • Biotherapeutic discovery and development
  • Molecule development
  • Antibody discovery and development

A workflow diagram highlighting the complementaryiQue® and Octet® systems for mAb screening characterization

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