CAR Construct Design and Screening
Chimeric Antigen Receptors (CARs) are engineered to specifically target cancer cell antigens and enhance T-cell proliferation and cytotoxicity. Target specificity and T-cell lifecycle are key factors in determining eventual therapeutic success.
After isolation of specific cell types from patient or donor` blood, T-cells are genetically modified ex vivo to express a CAR protein that recognizes specific tumor antigens. The goal of CAR construct engineering and screening is to overcome limitations such us toxicity, low antigen sensitivity and to achieve increased anti-tumor efficacy without compromising safety.
Complex biological interactions between CAR construct and tumor antigen require the utilization of orthogonal in vitro cell and protein analysis assays.
Octet®️ platforms, available using Bio Layer Interferometry (BLI) or Surface Plasmon Resonance (SPR), measures protein-protein interactions in parallel, without the use of detection agents. These robust approaches enable fast, real-time characterization of expressed proteins, even in complex and unpurified samples.
Featured applications include:
Related White Paper: Streamlining Affinity Analysis for Accelerated Lead Screening
The CellCelector platform is a unique and fully automated cell imaging and retrieval platform developed for detection, selection and isolation of single cells, clusters, spheroids and organoids as well as single cell clones and adherent colonies. In the field of development of CAR-T constructs the platform provides great solutions for:
Related Application Guide: Fully Automated Image-Based Single Cell and Colony Picking for Stem Cells
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Enables real-time, live-cell imaging within the stable condition of the incubator. Make informed decisions about your cultures, rapidly optimize and improve your workflows, plus study complex live-cell assays to fast track your next discovery!
Related eBook: Harnessing the Power of T Cells
Explore Live Cell Assays
Achieve fast, reliable, high-throughput analysis using minimal sample with the iQue® Advanced Flow Cytometry Platform with a portfolio of assays for adherent cells
Related White Paper: Understanding T Cell Phenotype and Function to Enable Improved Therapeutics
Explore Flow Cytometry
T-cell engineering includes different gene editing techniques which are sensitive reactions to nuclease activities. Arium® Ultrapure Water Systems produce ASTM Type 1 water, as well as RNase/DNase and endotoxin-free water to ensure consistent, high-quality results.
Related Application Note: Water is Life - Ultrapure Water in Cell Cultivation
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CellCelector represents the perfect tool for targeted isolation, picking and transfer of genetically modified cells with the desired genotype. The High-Throughput Nanowell-based Image Verified Cloning (HT-NIC) single cell cloning method with integrated monoclonality and viability proof enables industry leading outgrowth rates after clone transfer into 96 or 384 well plates.
Explore CRISPR Single-Cell Cloning
Octet® BLI software has a built-in epitope binning module to enable easy assay design, performance, and interpretation allowing for discriminating between antibodies that bind the same antigen but not the same epitope in cross-competition assays.
Explore Affinity & Kinetic Characterization
Relieve capacity bottlenecks throughout your entire experiment with the fastest plate sampling, integrated analysis, and novel data reduction tools. With the iQue® Advanced Flow Cytometry Platform, you can analyze a 96-well plate in as few as 5 minutes or a 384-well plate in as few as 20 minutes for a faster time to result.
Using Live Cell Analysis
Antibody and other protein therapeutics are a major focus in drug discovery pipelines today
Learn about streamlined in vitro assays used for phenotyping and functional assessment of modified CAR-T cells, with three case studies.
Reference: Rafiq, S., Yeku, O., Jackson, H. et al. Targeted delivery of a PD-1-blocking scFv by CAR-T cells enhances anti-tumor efficacy in vivo. Nat Biotechnol 36, 847–856 (2018). https://doi.org/10.1038/nbt.4195
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