Organoid Assay
Advances in human disease research, particularly in regenerative medicine and precision therapies, have been propelled by groundbreaking work using in vitro 3D cell culture models. These advanced models exhibit structural, morphogenetic, and functional properties that closely mimic in vivo pathophysiology, making them vital for predictive and translational research. However, to enhance organoid relevance to clinical outcomes, it is essential to reduce variability and increase insights.
Despite innovations in culturing ex vivo human models, challenges remain due to limitations in current technologies used to characterize these complex cell models:
The Incucyte® Organoid Assay overcomes these challenges through an optimized protocol and advanced solutions. Customers can choose to embed organoids in Matrigel or use our ready-to-use hydrogel 96-well nanowell plates. Combined with our software solution, this allows for real-time, label-free analysis, enabling efficient, reliable, multiparametric monitoring of organoids under physiologically relevant conditions. Drive organoid insights and novel therapy development with ease using the Incucyte® Organoid Analysis Software Module and the Incucyte® 3D Nanowell Plate.
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Learn more about Incucyte® Organoid Analysis Software
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Assess relevant data in 3D cell culture models through temporal, label-free image acquisition and analysis. The Incucyte® Organoid Assay uses an established protocol and the Incucyte® Organoid Analysis Software Module for continuous acquisition and objective analysis of organoid size, count and morphology in a microplate format. The Incucyte® Organoid Workflow supports the study of organoids via two different methods:
Track and standardize the analysis of organoids over time, to generate reproducible, physiological relevant data long-term, all from inside your incubator.
Additional Products
Qty.
Cat. No.
1 module
9600-0034-A00
Incucyte® SX5 Live-Cell Analysis System
1 instrument
4816
Simplify organoid workflow with easy-to-use Incucyte® 3D Nanowell plates – seed, culture, and treat organoids all within the same plate
See Example Data Below
Track organoids either in the Incucyte® 3D Nanowell Plate or embedded in Matrigel® to assess growth and morphology characteristics
Perform real-time, multiparametric analysis of organoids to better predict therapeutic outcomes using the Incucyte® Organoid Analysis Software Module
Figure 1. Incucyte® 3D Nanowell Plates are engineered to support high-throughput organoid studies essential for developmental biology, disease modeling, and drug discovery. Featuring hydrogel-printed microwells with unique pipetting ports, these plates enable uniform organoid formation and automated culture maintenance, enhancing efficiency and reproducibility in large-scale experiments.
Figure 2. Kinetically monitor distinct organoid phenotypes using the Incucyte® Live-Cell Analysis System. Mouse intestinal organoids were seeded as single cells (200 cells/µwell) into an Incucyte® 3D Nanowell Plate 500 µm. Brightfield video taken 0d post seeding show organoid formation, growth and maturation (0 – 10d post seeding).
Figure 3. Mouse intestinal, hepatic and healthy human brain organoids were seeded (50% Matrigel®) into 96-well plates and imaged in an Incucyte®. Brightfield videos taken 3d post seeding and images show cell type specific morphology & growth characteristics in 3D cell cultures.
Figure 4. Perform automated label free quantification of organoids. Brightfield images show mouse intestinal organoids (200 cells/µwell) grown in the Incucyte® 3D Nanowell Plate and treated for 5 days with Staurosporine (1 µM, STP), Camptothecin (1 µM, CPT), Cycloheximide (10 µM, CHX), and 5-FU (100 µM). Images and time-course data of Total Organoid Area and Organoid Darkness show the effects of the multiple compounds and control conditions over time.
Figure 5. Measure changes in organoid morphological features in response to compound treatment. Brightfield images show hepatic organoids (1K cells/well) ± protein kinase inhibitor Staurosporine (1 µM, STP). Vehicle treated organoids increase in size & number while a marked reduction is observed in the presence of STP. Images (zoomed in) and time-course data also demonstrate that in the presence of STP organoids lose distinctive rounded phenotype (increased eccentricity) and increase in darkness over time.
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High-quality, animal-free RUO growth factors and cytokines produced using recombinant DNA technology.
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